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The most common scoring system for critically ill patients is the Sequential Organ Failure Assessment (SOFA) score. Little is known about specific molecular signaling networks underlying the SOFA criteria. We characterized these networks and identified specific key regulatory molecules. We prospectively studied seven patients with sepsis and six controls with high-throughput RNA sequencing (RNAseq). Quantitative reverse transcription PCR (RT-qPCR) confirmation was performed in a second independent cohort. Differentially and significantly expressed miRNAs and their target mRNA transcripts were filtered for admission SOFA criteria and marker RNAs for the respective criteria identified. We bioinformatically constructed molecular signaling networks specifically reflecting these criteria followed by RT-qPCR confirmation of RNAs with important regulatory functions in the networks in the second cohort. RNAseq identified 82 miRNAs (45% upregulated) and 3254 mRNAs (50% upregulated) differentially expressed between sepsis patients and controls. Bioinformatic analysis characterized 6 miRNAs and 76 mRNA target transcripts specific for the SOFA criteria. RT-qPCR validated miRNA and mRNAs included IGFBP2 (respiratory system); MMP9 and PDE4B (nervous system); PPARG (cardiovascular system); AKR1B1, ANXA1, and LNC2/NGAL (acute kidney injury); GFER/ALR (liver); and miR-30c-3p (coagulopathy). There are specific canonical networks underlying the SOFA score. Key regulatory miRNA and mRNA transcripts support its biologic validity.

A novel dinucleating bis(pyrazolyl)methane ligand was developed for tyrosinase model systems. After ligand synthesis, the corresponding Cu(I) complex was synthesized and upon oxygenation formation of a μ-η2:η2 peroxido complex could...

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Many recent studies in numerical cognition have moved beyond the use of purely chronometric techniques in favor of methods which track the continuous dynamics of numerical processing. Two examples of such techniques include eye tracking and hand tracking (or computer mouse tracking). To reflect this increased concentration on continuous methods, we have collected a group of 5 articles that utilize these techniques to answer some contemporary questions in numerical cognition. In this editorial, we discuss the two paradigms and provide a brief review of some of the work in numerical cognition that has profited from the use of these techniques. For both methods, we discuss the past research through the frameworks of single digit number processing, multidigit number processing, and mental arithmetic processing. We conclude with a discussion of the papers that have been contributed to this special section and point to some possible future directions for researchers interested in tracking the continuous dynamics of numerical processing.