Objective:This study aimed to analyze RGD-Chitosan from Shrimp Shells' Scaffolds' (RCSSS) and CSSS membrane toxicity toward human dental pulp cells. Methods:Human dental pulp cells were cultured for 5 days and then exposed to RCSSS or CSSS membranes for 24 hrs. Cell viability was determined using an MTT assay method.Results: Cell viability of the RCSSS group and CSSS group was higher than the cell viability of the control group. The cell viability of the RCSSS group 2 mg (537.39%) was significantly higher than the CSSS group 2 mg (301.74%).Conclusions: RCSSS membranes were not toxic toward human dental pulp cells and showed better effect toward human dental pulp cells compared to CSSS membranes.

Methods:Human dental pulp cells were cultured for 5 days in Minimum Essential Medium Alpha (α-MEM) complete containing amphotericin B, penicillin, streptomycin, and fetal bovine serum. Then, the treatment group was exposed to crab shell chitosan RGD scaffold membrane and crab shell chitosan scaffold membrane incubated for 24 h. The toxicity of the crab shell chitosan RGD scaffold membrane was analyzed with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Result:The result of this research is that crab shell chitosan RGD scaffold membrane did not decrease the percentage of viability of human dental pulp cells. Conclusion:It is concluded that crab shell chitosan RGD scaffold membrane does not have toxic effects on human dental pulp cells.