Soil characterization plays an essential role in the water flow and availability of nutrients. In accordance with the above, this research sought to find a relationship between the soil slope, soil particle grain size distribution, infiltration rate, and the nitrate ion movement through the soil profile and in the water table of the Quebrada La Nitrera, located in a Colombian tropical nature forest reserve. It was observed that the slope and the rate of infiltration explain the nitrate concentrations from one site to another. Additionally, in April 2018, where on average there is more precipitation for the study area, the highest groundwater nitrate ions concentration (18 mg / L) was reported according to the standard value for natural waters (10 mg / L). This suggests that hydrological events also influence the transport of these minerals.