No abstract

Introduction: Housefly ( Musca domestica ), because of its body structure, filthy and feeding habits, is reported to be capable of carrying a variety of microbes, including potential pathogens, over its body appendages. Aims: This study aimed to investigate M. domestica in Calabar for pathogenic gut parasites, determine its role in parasitic diseases transmission, make appropriate recommendations toward its management and effective control of the associated parasitic diseases, and hence promote the society health status. Settings and Design: This was a cross-sectional study. Materials and Methods: A total of 300 M. domestica specimens (150 each from sanitary and insanitary areas, pooled in batches of five flies per pool) were used for the study. The parasites were detected from the spun deposits of fly body surface wash and gut contents using direct microscopy and formol-ether technique. Statistical Analysis Used: Data were analyzed using Statistical Package for the Social Sciences (SPSS), version 21.0, and Chi-square test at a significant level of P < 0.05. Results: Overall frequency of parasites detection was 46.7%, with helminths and protozoa showing similar frequency, 23.3% each. External body surface of flies recorded a statistically significant higher parasites frequency 76.7% than their gut contents 16.7% ( P = 0.000). A statistically significant higher parasite detection rate was observed in the insanitary (63.3%) than in sanitary areas (30.0%) ( P = 0.0114). The parasites detected in this study were Entamoeba histolytica/dispar 39.9%, hookworm (21.4%), Ascaris lumbricoides (17.9%), Trichuris trichiura (14.3%), and Giardia intestinalis (7.2%). Conclusion: This study has confirmed M. domestica in Calabar as a mechanical carrier of potential pathogenic gut parasites, especially in the insanitary areas, with E. histolytica/dispar being the most frequently encountered. Effective control of flies’ population in the human and animal habitats and increased public awareness on their health hazards are recommended.

Aim: Enumeration of bacterial counts is an important index of assessing the safety and quality of food products. This study aimed to investigate the occurrence of pathogenic bacteria in white shrimp samples obtained from major markets serving consumers in Calabar. Study Design: This study was a cross sectional study conducted between April 2017 and April 2018. Methodology: One hundred and twenty (120) samples of smoked dried Nematopalaemon hastatus (white shrimp) were analyzed for the presence of bacterial pathogens. Bacterial loads and identification of isolated organisms was determined using standard microbiological methods. Results: The results showed that 66.7% of the analyzed shrimps had aerobic bacterial counts exceeding the upper permissible limit (<1.0x106Cfu/g) and 56.7% had unsatisfactory (˃20Cfu/g) Vibrio counts. The study revealed the presence of different bacteria genera namely Klebsiella, Salmonella, Pseudomonas, Serratia, Vibrio, Citrobacter, Proteus, Aeromonas, Streptococcus, Escherichia, Coagulase-negative Staphylococci (CoNS), Enterobacter and Bacillus. Predominant organism was Salmonella spp (26.7%), followed by Vibrio spp (21.7%) while the least isolated organisms were Bacillus spp and Coagulase-Negative Staphylococci (1.7%) each. The occurrence of high counts of pathogens in seafood may cause food poisoning; especially in individuals who consume this seafood raw, or lightly or insufficiently cooked. Conclusion: Hence, seafood should be processed and packaged under standard hygienic conditions to reduce the risk of microbial contamination. In addition, public health awareness campaign targeted at consumers and vendors should be optimized with frequent monitoring by regulatory agencies.

No abstract