The stem of Akar Kuning is one of the natural ingredients that have the potential to treat various diseases such as diarrhea, fever, jaundice, diabetes, liver, skin infections, and as an antibacterial, so characterization is needed to become traditional medicine. The aim of this study was to determine the specific and nonspecific characterization of Akar Kuning stem extracts. Akar Kuning stems were extracted by maceration method in ethanol 70% solvent. The results of specific characterization showed organoleptic macroscopic test extracts which were yellowish-brown, blackish, thick, bitter, and distinctive. The value of water-soluble extract content of 50.47 ± 11.08% and soluble in ethanol 50.78 ± 5.53%. The results of the nonspecific characterization of the extract showed drying losses of 0.8082±0.033%, the water content of 15.6163 ±2.99%, the total ash content of 1.6455 ± 0.29%, the acid insoluble ash content of 2.1368 ± 0.91%, the specific gravity of the extract was 0.1053 ± 0.003 g/ml and did not have any residual solvents. Microbial contamination test results were 4.1 x 10 3 colonies/g while mold/yeast contamination was not found in the extract. In determining the metal content of Cd and Pb contamination in the extract is still below the maximum limit respectively <0.0043 mg/kg and 1.32 mg/kg.