A proteome reference map has been constructed for Vibrio cholerae El Tor, in the pI range of 4.0 to 7.0. The map is based on two-dimensional gels (2-D) and the identification, by peptide mass fingerprint, of proteins in 94 spots, corresponding to 80 abundant proteins. Two strains are compared, strain N16961 and a Latin American El Tor strain C3294. The consensus map contains 340 spots consistently seen with both strains grown in Luria-Bertani broth (LB) or minimal M9 medium. The results were obtained from nine gels run with 18 cm immobilized pH gradient strips and precast gels. The 2-D gels were anchored to real N16961 proteins identified by mass spectrometry. Various energy metabolism components and periplasmic ATP-binding cassette (ABC) transporter proteins were identified among the abundant proteins. Two isoforms of OmpU were found. Five operons are proposed and seven hypothetical proteins were experimentally confirmed. Comparisons are made with protein 2-D gels for a classical strain and to microarray analysis available for the N16961 El Tor strain. New results were obtained from the proteome analysis, indicating an abundance of periplasmic ABC transporter proteins not found in microarray studies.

The use of three-dimensional models is a strategy that facilitates teaching contents considered difficult for many high school teachers even in classes that do not have students with special educational needs. Our objective was to develop a tactile model that helps the understanding of the DNA structure and its replication. To achieve this, we adapted the steps proposed by Miotto for the production of tactile models. As a result, we constructed the matrix of the basic structures of the DNA molecule in cardboard covered with different textures. After approval by a blind student, who reported that the scale and textures chosen allowed identification of the structure as a whole and its parts, we prepared the high relief acetate plates. These were tested and approved by blind students in the "resource class" of a State Public School.