We needed effective and sustainable technologies for better microbiological control of crops, including Fusarium. However, photoluminescent UV-Vis methods are potential for diagnosing plant diseases with Fusarium. It has not been sufficiently studied despite the application of these methods for other biological researches. The excitation spectrum of the seeds during infection shifts to the shorter wavelength and a new maximum appears in the region λ ≈ 232 nm. The photoluminescence of infected seeds increases with excitation by radiation of wavelengths λ e,1 = 232 nm, λ e,2 = 362 nm and λ e,3 = 424 nm by 1.33-3.14 times, and λ e,3 = 424 nm-decreases by 1.1 times. Statistical moments μ 3 and μ 4 , asymmetry and kurtosis change only with short-wave excitation. When analyzing the decomposition of the frequency spectrum into Gaussian curves, the most informative ratio is the ratio of right-handed and left-handed Gaussians under excitation λ e,2 = 362 nm and λ e,3 = 424 nm. The ratios of their maxima change during infection by 1.36-3.2 times, and for excitation by radiation λ e,2 , the frequency boundaries of Gaussians change. The results of measurements and calculations provide a basis for the development of a method and device for photoluminescence diagnostics of fusarium seeds in UV-Vis ranges.