Endothelial dysfunction and inflammation are characteristics of subclinical atherosclerosis and may increase through progressive menopausal stages. Evaluating endothelial responses to acute exercise can reveal underlying dysfunction not apparent in resting conditions. The purpose of this study was to investigate markers of endothelial function and inflammation before and after acute exercise in healthy low-active perimenopausal (PERI) and late postmenopausal (POST) women. Flow-mediated dilation (FMD), CD31/CD42b and CD62E endothelial microparticles (EMPs), and the circulating inflammatory factors monocyte chemoattractant protein 1 (MCP-1), interleukin 8 (IL-8), and tumor necrosis factor-α (TNF-α) were measured before and 30 min after acute exercise. Before exercise, FMD was not different between groups (PERI: 6.4 ± 0.9% vs. POST: 6.5 ± 0.8%, P = 0.97); however, after acute exercise PERI tended to improve FMD (8.5 ± 0.9%, P = 0.09), whereas POST did not (6.2 ± 0.8%, P = 0.77). Independent of exercise, we observed transient endothelial dysfunction in POST with repeated FMD measures. There was a group × exercise interaction for CD31/CD42b EMPs (P = 0.04), where CD31/CD42b EMPs were similar before exercise (PERI: 57.0 ± 6.7 EMPs/μl vs. POST: 58.5 ± 5.3 EMPs/μl, P = 0.86) but were higher in POST following exercise (PERI: 48.2 ± 6.7 EMPs/μl vs. POST: 69.4 ± 5.3 EMPs/μl, P = 0.023). CD62E EMPs were lower in PERI compared with POST before exercise (P < 0.001) and increased in PERI (P = 0.04) but did not change in POST (P = 0.68) in response to acute exercise. After acute exercise, MCP-1 (P = 0.055), TNF-α (P = 0.02), and IL-8 (P < 0.001) were lower in PERI but only IL-8 decreased in POST (P < 0.001). Overall, these data suggest that perimenopausal and late postmenopausal women display different endothelial and inflammatory responses to acute exercise.

The Daily Mile (TDM) is a school-based physical activity intervention encompassing a 15-minute run at least three times per week. This study aimed to determine (1) the effects of performing TDM for 12 weeks on Dutch primary school children’s aerobic fitness levels and (2) if additional personal support for teachers impacted the effectiveness of TDM. Nine Dutch primary schools (n = 659 children, grades 5–8) were allocated to a control (no TDM), intervention (12 weeks TDM) or intervention-plus (12 weeks TDM, additional personal support) group. The Shuttle Run Test (SRT) was used to assess aerobic fitness at baseline and follow-up. Data were analyzed using a multiple-imputed dataset and multilevel linear regression models to account for the clustering of students within classes and classes within schools. The regression analyses were adjusted for sex and age. Compared with the control group, significant intervention effects of TDM on SRT score were observed for the intervention group (β = 1.1; 95% CI: 0.8; 1.5) and the intervention-plus group (β = 0.6; 95% CI 0.3; 0.9). Additional personal support had no impact on the effectiveness of TDM. These results suggest that performing TDM at least three times per week for approximately 12 weeks increases primary school children’s aerobic fitness. Additional personal support did not improve the effectiveness of TDM on aerobic fitness within this period. These results contribute to the body of evidence surrounding TDM, but further research is needed regarding long-term implementation of TDM.

Background The Daily Mile is a school-based physical activity intervention that aims to improve children's aerobic fitness levels. It encompasses a 15-minute run for the whole class on or around the school grounds at least three times per week. The Daily Mile is an easy and accessible intervention, but the downside is a low threshold to stop or skip a session. Therefore, we aimed to determine 1) the effects of performing The Daily Mile for 12 weeks on the aerobic fitness levels of Dutch primary school children and 2) if additional personal support for teachers impacted the effectiveness of The Daily Mile. Methods We conducted a controlled trial in grades 5 through 8 of nine primary schools across the Netherlands. Schools were allocated to control, intervention (12 weeks The Daily Mile) or intervention-plus (12 weeks The Daily Mile and additional support) group. Children completed the shuttle-run test (SRT) at baseline and follow-up, 12 weeks apart. We analyzed the data using multi-level linear regression models clustered within individuals and by classes and schools. All models were adjusted for sex and age. Results We collected complete data sets for 536 children (mean age 10.0 years). The participation rate of classes in the intervention group was 87.8% and in the intervention-plus group 89.7%. After correcting for age and sex, the adjusted model showed a significant intervention effect on SRT-score after 12 weeks for both the intervention group (1.1 stages; 95% CI 0.75, 1.47) and the intervention-plus group (0.6 stages; 95% CI 0.32, 0.89), when compared to the control group. Conclusion Performing The Daily Mile at least three times per week for a 12 week period can be effective in increasing the aerobic fitness levels of primary school children. Additional personal support for teachers did not improve the effectiveness of the intervention on aerobic fitness within this time frame. This may be due to the high implementation rates in both the intervention group and the intervention-plus group. Possibly additional personal support might become beneficial for maintenance on the longer term.

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