Background: Burn is considered one of the most dangerous domestic home injuries in early childhood stage that causes different harmful effects. Objective: The current study aimed to evaluate the effect of health education program for mothers to prevent burn in early childhood. Subjects and methods: Design: A quasi experimental design was conducted. Setting: The study was conducted at Maternal and Child Health Center in Menouf City. Sample: Convenience sample of 167 mothers from the previously mentioned Maternal and Child Health Center. Tools: Structured interview questionnaire composed of three parts; mothers demographic Characteristics, mothers' knowledge about burn, and mothers' reported practices to prevent burn at early childhood. Results: Before program implementation about 60% of mothers had poor knowledge score about burns. On the other hand, 90% of mothers have good knowledge score at immediate post health education program implementation (P= 0.001). Only 30% of mothers reported done practice before program, and improved to 70% after the health educational program (P= 0.001). Conclusion: Continuous health educational programs for mothers to enhance their knowledge and practice about burn prevention are needed.