Background and aims: Down's syndrome patients are suffering from such gastrointestinal and metabolic complications which affect their prolongation for survival and this could be attributed to the malnutrition system. To reduce the risk factors for mortality, the paper has focused on the assessment of the socioeconomic, clinical, physical, biophysiological, and biochemical characteristics of them which can be affected by the type of nutrition system, toxicity, and ecological footprint.Methods: Patients were males with trisomy 21 diagnosed by karyotype test and assessed by clinical examinations. The clinical observations, medical interventions, and oral diseases associated with DS have been defined and oral treatment is explored. Samples collected from different biofluids. The physicochemical analyses of the biomatrix samples were performed and these properties had compared to findings of healthy males and age-matched controls. In specific, trace elements which could be originated from environmental resources were assessed in saliva, blood, urine, and hair.Results: Duraphat application was proved effective for their oral treatment and saliva was the optimum biomarker for detecting malnutrition. The patients were hypersensitive to Cu while the Mn content in blood and hair was considered an expression to the degree of epileptic condition and chronic seizure development. The ecological footprint was 5.6 gha and carbon footprint was recognised in food poverty habits. These can be reduced by eating more plant-based proteins and fibre-rich foods with low saturated fats and sodium.Conclusions: The current findings provide an up-to-date reference for expected developmental outcomes in children with DS in terms of biophysicochemistry. The genetically sensitive intervention is affected by heredity factor and sensitivity to toxics. Down's syndrome is encouraged to live green-hipster life. Besides, doctors are recommended to order the physicochemical analyses for early detection of this special intervention.WHO Clinical Trial Registry: Study ID number: DRKS00014074 Title page of the study: Down's syndrome in children males – biochemical characterisations in different media using non-randomised trial and systematic study Website of Clinical Trial Registry: http://www.drks.de/drks_web/navigate.do?navigationId=trial.HTML&TRIAL_ID=DRKS00014074

Background Down's syndrome patients are suffering from such gastrointestinal and metabolic complications which affect their prolongation for survival and this could be attributed to the malnutrition system. To reduce the risk factors for mortality, the paper has focused on the assessment of the socioeconomic, clinical, physical, biophysiological, and biochemical characteristics of them which can be affected by the type of nutrition system, toxicity, and ecological footprint. Methods Patients were males with trisomy 21 diagnosed by karyotype test and assessed by clinical examinations. The clinical observations, medical interventions, and oral diseases associated with DS have been defined and oral treatment is explored. Samples collected from different biofluids. The physicochemical analyses of the biomatrix samples were performed and these properties had compared to findings of healthy males and age-matched controls. In specific, trace elements which could be originated from environmental resources were assessed in saliva, blood, urine, and hair. Results Duraphat application was proved effective for their oral treatment and saliva was the optimum biomarker for detecting malnutrition. The patients were hypersensitive to Cu while the Mn content in blood and hair was considered an expression to the degree of epileptic condition and chronic seizure development. The ecological footprint was 5.6 gha and carbon footprint was recognised in food poverty habits. These can be reduced by eating more plant-based proteins and fibre-rich foods with low saturated fats and sodium. Conclusion The current findings provide an up-to-date reference for expected developmental outcomes in children with DS in terms of biophysicochemistry. The genetically sensitive intervention is affected by heredity factor and sensitivity to toxics. Down's syndrome is encouraged to live green-hipster life. Besides, doctors are recommended to order the physicochemical analyses for early detection of this special intervention.