Микробиология и вирусология 155 УДК 579.852.11:616.981.455; 577.151.45 А.в. корнева, в.б. Николаев, с.Н. козлов, Е.Ю. Марков, А.в. Мазепа, Ю.о. Попова выявление мембрАносвязАнных протеАз FRaNCISElla tUlaRENSIS ФКУЗ «Иркутский научно-исследовательский противочумный институт Сибири и Дальнего Востока» Роспотребнадзора, Иркутск, Россия Проведено исследование наличия протеолитической активности и состав протеаз в поверхностных структурах клеток шести штаммов Francisella tularensis разных подвидов. Препараты мембранной фракции возбудителя туляремии получали путем обработки микробной массы мочевиной. Субстратный электрофорез выявил три общих для всех штаммов полипептида, обладающих протеолитической активностью. У препаратов F. tularensis subsp. nearctica и F. tularensis subsp. novicida обнаружены дополнительные полосы протеаз. Ключевые слова: Francisella tularensis, субклеточные фракции, наружные мембраны, ферменты, протеазы протеолитическая активность dEtEction of mEmbranE-bound protEasEs of FRaNCISElla tUlaRENSIS Sterile preparations of membrane fractions were prepared by processing of live Francisella tularensis cells of different subspecies with 4.5 M urea solution and differential centrifugation. For the first time, proteolytic activity was detected and studied by tests of radial enzyme diffusion and substrate polyacrylamide gel electrophoresis using gelatin as a substrate. Spectrum of gelatinases in the resulted preparation were detected. Quantitative inter-strain differences in the protease activities and their qualitative composition in membrane preparations of various virulent F. tularensis strains was analyzed. Avirulent F. tularensis 21/400 subsp. holarctica (I-214) strain demonstrated the greatest gelatinase activity in enzyme diffusion method and the lowest hydrolytic activity was seen in F. tularensis B-399 A-Cole subsp. tularensis (I-386) and F. tularensis Utah 112 subsp. novicida (I-384), other preparations showed intermediate activity. Enzyme electrophoresis in the protease spectra determined the presence of proteins with proteases activity 50-100 kDa, and in the spectrum preparations of F. tularensis I-386 and I-384 were detected additional bands of proteases.

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The article presents the results of the optimization of breast screening based on microwave radiothermometry due to the development of a test system for express diagnostics of malignant breast tumors and identification of female patients at risk. The current program of investigation involves a time-consuming history taking and a preliminary measurement of the temperature at the control points. The purpose of the research was to find out is there a relationship between the presence or absence of positive an-swers to anamnestic questions of the program and changes of temperature of the breast. Another task was to determine the degree of dependency of temperature changes in the mammary gland on the diameter of the gland and the temperature in the axillary area and other control points. Three groups of female patients were examined: healthy subjects, risk group (with benign nodules) and breast cancer patients. In the group of cancer female patients the temperature data of healthy mammary glands were studied separately. The results showed that the temperature changes depend on the age and the diameter of the gland only for healthy subjects. The temperature in the axillary area and the control points of the study has a high degree of direct correlation with the temperature changes in the mammary gland in all groups of female patients.