New stability indicating reverse phase high performance liquid chromatography (HPLC) method is developed for the determination of Imiquimod and its impurities. Separation is achieved on a C18 column (Inertsil-ODS3 4.6 × 250 mm, 5 µm) using gradient elution mode with mobile phase-A having disodium hydrogen phosphate buffer (10 mM) with 0.1% v/v triethyl amine and pH adjusted to 6.0 with ortho-phosphoric acid while mobile phase-B consist of an equal mixture of methanol and acetonitrile. The flow rate was optimized to 1.2 mL/min and column oven temperature 30°C. Detection was carried out at wavelength 226 nm. This developed method is then validated as per International Council for Harmonisation (ICH) guideline and found out to be linear, accurate, specific, selective, precise, and robust. The drug is also subjected to forced degradation using Original Research Articlestress conditions of acid-base hydrolysis, oxidation, photolysis and thermal degradation. Considerable degradation was found out only in harsh condition of oxidative degradation where degradation impurity is also predicted. All degradation products were well separated. Test solution was found to be stable for 24 hrs. The method can be successfully applied for the determination of Imiquimod and its impurities in routine and stability samples.

No abstract

Objective: Black plum seed has unique medicinal value and used as antidiabetics, antibacterial, and anti-inflammatory. Methods: Gas chromatography-mass spectrometry (GC-MS) analysis of the extracts of black plum seed obtained using solvent extraction with hexane as a solvent is used to attempt the identification of prominent components. Results: Black plum seed extract is obtained by solvent extraction technique using Soxhlet extractor. A total of 10 compounds are predicted in black plum seed extract by GC-MS analysis. Conclusion: The work presented relates to the study of GC-MS analysis of the extract of black plum seed obtained using solvent extraction with hexane as a solvent. Of ten compounds of black plum seed extract, five compounds are known to have antimicrobial properties.

Morpholinium glycolate was found to be highly potent and reusable ionic liquid as a catalyst for the synthesis of dihydropyrano[2,3-c]pyrazol-5-carbonitriles via a one-pot multicomponent condensation reaction of aryl aldehydes with ethyl acetoacetate, hydrazine hydrate, and malononitrile in an aqueous medium at room temperature. The key advantage of this present protocol is an economical, excellent yield, shorter reaction time, easy work-up, no chromatographic purification and the catalyst can be effortlessly recovered and reused in several runs.

Copper oxide nanoparticles were prepared by five sol-gel methods. The comparative study was made by the characterization such as FTIR, SEM-EDX, XRD. Out of these protocols, method five is more suitable and gives more accurate and appropriate results. From the data of EDX it is also found that method five gives higher yields of copper oxide nanoparticles. The efficiency of these nanoparticles as low cost adsorbent for the removal of methylene blue (MB) capacity from aqueous solutions was investigated. The effect of different parameters such as amount of adsorbent, concentration of dye, contact time, pH, temperature were studied the adsorption capacity and adsorption behavior of nanoparticles under various conditions. The experimental isotherm data has been studied. The kinetic study of the adsorption obeys Pseudo-first order model. The thermodynamic parameters namely Gibbs free energy, enthalpy, and entropy have revealed that the adsorption of methylene blue on the nanoparticles are feasible, spontaneous and exothermic.