A rapid and highly sensitive reversed phase high performance liquid chromatographic method has been developed for quantitative estimation of omeprazole in pharmaceutical preparations. The method has been validated according to FDA and USP guidelines with respect to accuracy, precision, specificity and linearity. The method was developed by using a gradient condition of mobile phase comprising 90% aqueous acetonitrile to 100% acetonitrile for 10 minutes at a flow rate of 0.7 mL/min over C-18 (ODS, 250 x 4.6 mm) column at ambient temperature. More than 97% recovery demonstrated the accuracy of the protocol. Intra-day and inter-day precision studies of the new method were less than the maximum allowable limit (RSD% ≤ 2.0 according to FDA). The method showed linear response with correlation coefficient (r 2 ) value of 0.998. Therefore, it was found to be accurate, reproducible, sensitive and less time consuming and can be successfully applied for the assay of omeprazole formulations.

Language choices in colonized societies are shaped by a combination of local, national, and global forces as well as historical, political, religious and economic factors. This is particularly true in Dhaka and Cox's Bazar. Adopting an ethnography, this study attempts to investigate the intersection of language practices and ideologies by examining the language use and language choices displayed in cultural heritage sites and tourism scenic spots both in public and private multilingual signs. Data are collected through linguistic signs represented at the two places and individual interviews with local people. Findings indicate that multidimensionality marks the linguistic landscape in Bangladesh. The sociopolitical dimension signifies the officially laid-down monolingual Bangla-oriented policies, which accentuate compulsory use of the national language Bangla standing for Bangladeshi nationalism and identity. English as a post-colonial reproducer of linguistic hegemony is presented in various aspects in Bangladesh. The economic dimension is manifested in the prominent use of Chinese as a newly emerging foreign language and the employment of Arabic, which is a symbol of Bangladeshi main religion Islam. The study provides a new context for understanding Bangladesh's multilingual practices and its language planning and management in the context of globalization.

A rapid and sensitive isocratic reversed phase high performance liquid chromatographic method has been developed for quantitative analysis of metformin hydrochloride and gliclazide in bulk as well as pharmaceutical dosage forms. The method was validated according to FDA, ICH and USP guidelines with respect to accuracy, precision, specificity and linearity. For metformin, the method was developed by using the mobile phase comprising of sodium dihydrogen phosphate solution (NaH 2 PO 4 ) (pH 3.0) and acetonitrile (90:10, v/v) at a flow rate of 0.5 mL/min over C 18 bonded silica column (3.9 x 300 mm, 5 µm) at ambient temperature. On the other hand, the same mobile phase was used in the ratio of 20:80 (v/v) at a flow rate of 0.6 mL/min for gliclazide. The recovery was found to be more than 97% for metformin and 102% for gliclazide that demonstrated the accuracy of the protocol. Intraday and interday precisions of the new method were less than the maximum allowable limit (%RSD 2.0) specified by the USP, ICH and FDA. The method revealed linear response with correlation coefficient value of 0.999 in both cases.

The methanol extracts and their pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates of Ophirrhiza mungos, Mussaenda macrophylla, Gmelina philippensis and Synedrella nodiflorawere subjected to assays for membrane stabilizing and thrombolytic activities. The extractives inhibited heat- as well as hypotonic solution-induced haemolysis of human erythrocytes in vitro. The pet-ether soluble fraction of O. mungos, M. macrophylla and S. nodiflora demonstrated 61.16 % & 24.75%, 52.55% & 23.35% and 60.24% & 22.85% inhibition of hemolysis of RBC caused by hypotonic solution and heat, whereas the carbon tetrachloride soluble fraction of G. philippensis showed 49.05% and 21.25% inhibition of hypotonic and heat induced hemolysis of RBC, respectively. Here, acetyl salicylic acid was used as reference standard at 0.10 mg/mL. Among the four plants, the carbon tetrachloride soluble fraction of O. mungos, methanol extract of M. macrophylla, carbon tetrachloride soluble fraction of G. philippensis and chloroform soluble fraction of S. nodiflora revealed highest thrombolytic activity with clot lysis value of 50.09%, 49.50%, 47.14% and 46.37%, respectively. Standard streptokinase and water were used as positive and negative controls which showed 65.00% and 3.84% lysis of clot, respectively.

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