The main object of this paper is to study doubly stochastic matrices with majorization and the Birkhoff theorem. The Perron-Frobenius theorem on eigenvalues is generalized for doubly stochastic matrices. The region of all possible eigenvalues of n-by-n doubly stochastic matrix is the union of regular (n – 1) polygons into the complex plane. This statement is ensured by a famous conjecture known as the Perfect-Mirsky conjecture which is true for n = 1, 2, 3, 4 and untrue for n = 5. We show the extremal eigenvalues of the Perfect-Mirsky regions graphically for n = 1, 2, 3, 4 and identify corresponding doubly stochastic matrices. Bearing in mind the counterexample of Rivard-Mashreghi given in 2007, we introduce a more general counterexample to the conjecture for n = 5. Later, we discuss different types of positive maps relevant to Quantum Channels (QCs) and finally introduce a theorem to determine whether a QCs gives rise to a doubly stochastic matrix or not. This evidence is straightforward and uses the basic tools of matrix theory and functional analysis.