The present study was undertaken to investigate the antioxidant and anti inflammatory activity of ethanolic extract of Glochidion acuminatum leaves. Ethanolic extract of the leaves of the plant was subjected to various in-vitro assays. Antioxidant potential was tested through total phenol content, total flavonoid content, DPPH free radical scavenging capacity and total anti-oxidant capacity assay. Anti-inflammatory activity was tested against the denaturation of protein. Glochidion acuminatum exhibited good antioxidant potential on total phenol content assay, TPC as GAE was 507.24±19.48mg/gm. It showed remarkable antioxidant potential on total flavonoid content assay, TFC as QE was 486.28±16.44mg/gm. In DPPH free radical scavenging assay IC50 value was 14.97μg/ml while for standard ascorbic acid it was found to be 8.87μg/ml which express potent antioxidant activity. It also showed excellent antioxidant potential on total antioxidant capacity assay, TAC as AAE was 712.5±5.89μg/ml. Anti-inflamatory assay showed that the plant leaves extract possess very good anti-inflamatory potential. These effects may be due to the presence of different phytoconstituents present in the extract. Further studies are required to evaluate these effects and due to the findings which warrants further more sophisticated research.