We investigated the effects of two commercial diatomaceous earth based insecticides (DE), Protect-It Ò and SilicoSec Ò , the nano-structured silica product AL06, developed by the section for Urban Plant Ecophysiology at Humboldt University Berlin, and the monoterpenoids, eugenol, and cinnamaldehyde on two stored product pests, Callosobruchus maculatus and Sitophilus oryzae. ProtectIt Ò was more effective than SilicoSec Ò against C. maculatus while the reverse was true for S. oryzae. Generally C. maculatus was more sensitive towards DE and silica treatment than S. oryzae. Mortality rate of both pest species increased when DE's were applied to food commodities previously treated with a monoterpenoid. In admixture experiments, the toxicity of SilicoSec Ò ? cinnamaldehyde (LD 50 = 42.73 ppm), SilicoSec Ò ? eugenol (LD 50 = 24.30 ppm), and Protect-It Ò ? eugenol (LD 50 = 2.60 ppm) was increased over DE alone against S. oryzae. Both substances showed a synergistic effect considering their co-toxicity coefficient relative to the LD 50 -value. In contrast, we could not find any synergistic effects in experiments with C. maculatus. Here only Protect-It Ò ? cinnamaldehyde (LD 50 = 20.84 ppm) showed an additive effect while all other combinations of monoterpenoid and DE indicated antagonistic effects. In addition to contact insecticidal effects both monoterpenoids showed a strong fumigant action. The presented results indicate that the natural product DE has great potential to replace synthetic pesticides commonly used in stored product pest management. Efficacy of DE can be improved by adding certain monoterpenoids against certain insect pests.
The biological activity of essential oil extracted from coriander, Coriandrum sativum L. (Apiaceae) against eggs, larvae and adults of Tribolium castaneum (Herbst) was investigated in a series of laboratory experiments. Bioassays conducted in air-tight glass chambers showed vapour toxicity and strong repellency on Wlter paper arena test towards all the stages used. Responses varied signiWcantly (P < 0.001) within insect stages, dosages and exposures. At 12.0 g/ml dosage, mortality of adults reached 95%, but for 12-, 14-and 16-days larvae, mortalities were 65, 74 and 82%, respectively. On the developmental inhibition, individuals fumigated at the larval stage conWrmed that the percentage of larvae reaching to pupal stage and pupae to adult stage, decreased signiWcantly (P < 0.001) with increasing dosage concentration. The oil fumigation yielded 100% mortality for T. castaneum larvae, pupae and adults at 0.08 g/ml dosage. The oil had fumigant activity against eggs and the toxicity progressively increased with increased exposure times and concentrations. One hundred per cent T. castaneum egg mortalities were obtained with oil fumigation at 20 g/ml and 96 h exposure period. C. sativum oil also showed highly repellent activity to the adults of T. castaneum, with overall repellency was 90% and at a dosage of 12 g/ml repellency was 100% in a Wlterpaper arena test.
With the phase-out and impending ban of methyl bromide, sulfuryl fluoride is among the most promising alternative fumigant insecticides for control of stored-product insect pests. It has been evaluated for control of several stored-product insect pests, but there are few data available on its efficacy for control of stored-product psocids (Psocoptera). We evaluated sulfuryl fluoride for control of different life stages of the psocids Liposcelis paeta Pearman, L. entomophila (Enderlein), L. bostrychophila Badonnel, L. decolor Pearman, and Lepinotus reticulatus Enderlein (Trogiidae) in 48-hr trials at 27.5 degrees C. Adults and nymphs were susceptible to sulfuryl fluoride. Complete (100%) adult and nymphal mortality was recorded at concentrations between 4 and 8 g/m3, except for L. decolor for which all adults were only killed at 24 g/m3. Eggs were tolerant to sulfuryl fluoride. Complete egg mortality was achieved at 24 and 72 g/m3 for L. reticulatus and L. decolor, respectively. Survival of L. paeta eggs was recorded even after exposure to 96 g/m3. Given that the highest United States label concentration for sulfuryl fluoride for a 48-h exposure interval is 31.25 g/m3, our study indicates that high doses and/or longer exposures are needed for complete mortality of eggs of L. decolor and L. paeta. Moreover, the present work suggests that there is considerable variation in efficacy of sulfuryl fluoride for control of different psocid species.
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