The present study was conducted to know the status of biosecurity and prevalent parasitic diseases in finfish hatcheries of Jessore district. The peak season of seed production was found April to May. Management of biosecurity has been practiced to prevent disease outbreaks and exert quite satisfactory. It was observed; hatchery owners cleaned their hatchery units regularly and maintained hygiene (76.66%), water quality (56.66%), disinfected equipments (76.00%), stocked disease free broods (76.00%) and quarantine (56.66%). Prevalence of diseases were- lernaeasis (34.10%), argulosis (23.86%), leeches (11.36%), dactylogyrosis (7.95%), gyrodactylosis (10.23%) and others (12.50%) in brood fish and fry. In broods, average prevalence was 16.67% with 9.25% mortality. Besides average prevalence was 10-15% with 10% mortality in fry. The epizootiological pattern showed the highest frequency of parasitic diseases during winter because of loss of appetites. The study demonstrated that sumithion was used by (93.32%), magic (46.33%), depterax (56.67%), lime with KMnO4 (80.00%), lime with salt (66.67%) and lime- salt- KMnO4 (50.00%) by hatchery owners respectively for treatments. Lack of assistance, proper knowledge and suitable therapeutics with its proper use were the major problems in the hatcheries.