Background: Siddha Makardhwaja (SMD) is a classical Ayurvedic formulation markedly used as a traditional medicine in the rural population for various purposes such as stimulant, tonic, and rejuvenator.Methods: The present study is conducted to evaluate the effect of conventionally prepared SMD on the hematological parameters in experimental animals, for providing scientific data base for its logical use in clinical practice. Acute toxicity tests were conducted to determine the LD50 of the drug. To find out the effect of chronic administration of SMD on hematological parameters it was administered chronically to the male Sprague-Dawley rats at a dose of 40mg/kg for 28 days.Results: In this experiment the TC, DC, various erythrocytic parameters, platelet parameters, ESR were determined. The results of the studies are given below. There is an (13.41%) increase in the number of white blood cell count of the male rat, the increase though not significant yet it was prominent (p=0.257). There is an (15.87%) increase in the absolute count of Neutrophils of the male rat, the increase though not significant yet it was prominent (p=0.371). There is an (12.29%) increase in the absolute count of Lymphocytes of the male rat, the increase though not significant yet it was prominent (p=0.388). There is a statistically significant (p=0.035) increase in the number of platelet count of the male rat (11.13% increase). There is a (2.03%) decrease in the platelet volume distribution width of the male rat, the decrease though not significant yet it was noticeable (p=0.094). There is a statistically insignificant (p=0.619) (10.0%) increase in Erythrocyte sedimentation rate in blood from the male rat.Conclusions: As SMD decreases and increases abnormally on the hematological parameters in body of treated rats, so it should not be administered chronically at a higher dose. Further studies should be done by reducing the administered dose.

Chandraprabha Batika (CPB) is an Ayurvedic preparation that is used in the rural population for different purposes as traditional medicine. In this study, the effect of CPB on thyroid hormone profile was evaluated after chronic administration of this drug to male Sprague-Dawley rats. The acute pharmacological test of CPB recorded no death or any signs of toxicity even at the highest dose of 4,000 mg/kg body weight. For chronic pharmacological evaluation, the animals were divided into two groups. The first group was given CPB preparation at a dose of 40 mg/kg body weight for 28 days while the second group that served as the control received water for the same period. After 28 days of chronic administration of the CPB preparation, the following effects on the thyroid hormone panel were noted: a statistically insignificant decrease in the serum circulating total thyroxine (tT4) and total triiodothyronine (tT3) levels of the male rats; a decrease in the serum circulating free thyroxine (fT4) level of the male rats, the increase though not significant yet it was prominent; a statistically insignificant increase in the serum circulating free triiodothyronine (fT3) level of the male rats; an increase in the serum circulating thyroid-stimulating hormone level of the male rats, the increase though not significant yet it was prominent.