Background and Objective: Strawberry leaf spot diseases caused by fungi are the most common and economically significant concern, reducing strawberry productivity and quality. Current investigation attempts to find out the causal agent of certain leaf spot disease of strawberry, growth behaviour of the pathogen and its in vitro control strategies. Materials and Methods: A fungal pathogen causing leaf spot of the strawberry plant was isolated from a distinguish symptom and identified as Neopestalotiopsis chrysea (Maharachch. and K.D. Hyde) Maharachch., K.D. Hyde and Crous. Through classical taxonomy and molecular characterization based on sequencing the internal transcribed spacer region of rDNA of the fungus. The growth behaviour of the fungus was evaluated on six different solid fungal culture media, different light, temperatures and pH conditions. Dual culture and food poison techniques were applied to evaluate the efficacy of Trichoderma spp. and chemical fungicides against the fungus, respectively. Results: The optimum mycelial growth of the fungus was recorded on potato dextrose agar and potato sucrose agar media, at 20EC temperature, pH 7 and under complete dark conditions. Trichoderma reesei exhibited significant mycelial growth inhibition of the fungus. The maximum mycelial inhibition of the fungus was found in amistar top (100 ppm), followed by tilt 250 EC (500 ppm). Conclusion: To the best of our search, the leaf spot disease of strawberry caused by Neopestalotiopsis chrysea is a new record in Bangladesh.

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