Worldwide a lot of people suffering from massive burns, skin defects/diseases and surgical wound associated skin dysfunctions each year require rapid amniotic grafts. Amniotic Membrane (AM) has been employed in the treatment of wounds for almost 100 year; beginning with early application of AM obtained from labor and delivery to various types of burns and wounds. Amniotic membrane is rich in collagen and growth factors that support the healing process. The avascular, low immunogenic, anti-inflammatory, anti-scarring and wound healing properties of amniotic membrane make it valuable in a wide range of regenerative medicine applications. Nowaday, AM has become an important source of stem cell as well. Amniotic membrane is a promoter of epithelialization and is a non-tumorigenic tissue and its use has no ethical problems. Because of its attractive properties, AM has been applied in several surgical procedures related to ocular surface reconstruction and the genito-urinary tract, skin, head and neck, among others. So far, the best known and most auspicious applications of AM are ocular surface reconstruction, skin applications and tissue engineering. Here, we provide an overview of the properties and therapeutic potential of AM.