Fusarium wilt, caused by Fusarium oxysporum f.sp. cubense (Foc), is one of the most destructive diseases of banana and has spread in many plantation areas in Indonesia. Until today, the effective ways to control banana fusarium wilt disease have not yet been found. Some studies indicated that Allium spp. could be used to suppress plant diseases caused by Fusarium. Allium spp. are important horticultural crops which are generally cultivated in some areas in Indonesia. This research was conducted to determine the effect of several species of Allium spp. intercropped with banana to improve soil suppressiveness against banana fusarium wilt disease. The results showed that up to 12 months after planting, from 3 species of Allium spp. (A. tuberosum/Chinese leek, A. fistulosum/bunching onion, and A. cepa var. aggregatum/shallot) intercropped with banana Ambon Kuning (AAA) cultivar, Chinese leek and shallot were able to suppress the incidence of fusarium wilt disease of banana by 46 and 33% respectively. Soil analysis on the rhizosphere of banana intercropped with Chinese leek and shallot had lower population of total Fusarium compared to the other treatments. Analysis of fluorescein diacetate (3'.6'-diacetylfluoerescein) or FDA also showed that total microbial activity in the rhizosphere of banana intercropped with Allium spp. was also lower compared to control treatment (without intercopping). The observation of the effect of Allium spp. extracts on Foc showed that Allium spp. extracts were able to suppress the development of the colony and spore germination of Foc in vitro.

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Penyakit layu fusarium pada pisang disebabkan oleh Fusarium oxysporum f.sp. cubense (Foc). Foc merupakan salah satu patogen tular tanah. Intensitas serangan penyakit di lahan berbeda-beda, lahan dengan intensitas sangat parah disebut tanah kondusif. Tanah supresif dapat menekan dan mengontrol laju perkembangan patogen tular tanah. Penelitian ini bertujuan untuk mengetahui sifat fisika dan kimia tanah supresif dan kondusif terhadap penyakit layu fusarium pisang serta mengetahui efek dan kadar hara dari penambahan pupuk kandang dan silika terhadap penyakit layu fusarium pisang. Sampel tanah supresif berasal dari Sarampad, Cianjur dan tanah kondusif berasal dari Salaman, Magelang. Tanaman pisang kultivar Ambon Kuning digunakan untuk mengetahui pengaruh pupuk kandang dan silika terhadap layu fusarium. Analisis sifat kimia menunjukkan tanah supresif memiliki pH netral, kelas tekstur geluh pasiran, N total, P total, C organik, bahan organik, Si tersedia yang lebih tinggi dibandingkan tanah kondusif. Penambahan pupuk kandang dan silika dapat menekan laju perkembangan dan pertumbuhan penyakit layu fusarium pada tanah supresif. Setelah perlakuan kandungan N total, K total, bahan organik dan C organic pada tanah supresif lebih tinggi.

The research aims to produce a textbook for undergraduate students, especially the students of the Agrotechnology Study Program in Agricultural Faculty of UPN "Veteran" Jawa Timur. Since beginner students quite hard to understand the theory and practice of biotechnology in the agricultural field, thus a textbook was needed to help them improved their understanding of all the general and basic knowledge of biotechnology. This research was conducted by collecting any information from some literature due to biotechnology, then write it into a good textbook. Moreover, some of the research results were also reported in it. This textbook was included eight chapters, respectively: 1) definition, history, and the role of biotechnology; 2) genetic materials; 3) gene structure and expression; 4) molecular technique; 5) biotechnology application; 6) molecular marker and biomarker; 7) bioinformatic; and 8) security, management and intellectual property rights of the genetically modified organism. This book was also written completely with some questions as a task for the students in each chapter. As a result, a textbook with the specificity is 205 pages in HVS (A4) size, written in Bahasa, and the title of this book is "Bioteknologi Umum Untuk Mahasiswa Pemula (Teori dan Aplikasi Bioteknologi di Segala Bidang)". Although this book was aiming at agricultural students, the theory in it was also enriched with biotechnology information in some aspects, such as medical, environmental, and food technology.

The use of organic fertilizers can improve soil conditions. Soil conditions include soil structure and health. The soil structure that has good aeration and drainage can increase plant productivity. The content of organic matter and microorganisms in healthy soil can be beneficial for sustainable agriculture. Making organic fertilizers can take advantage of the remains of organic matter or dry leaf litter around the environment. Appropriate application of organic fertilizers can affect plant growth and development as well as the quality of crop yields. Plant growth and development can affect plant productivity. High plant productivity and good quality yields can increase the selling value of the crop. The selling value of high yields can increase income and increase the welfare of farmers. Food crops such as vegetables can use organic fertilizers so that vegetables become healthier. Farmers can also reduce spending on inorganic fertilizers or chemical fertilizers. Making organic fertilizers can also be a business opportunity for farmers. The purpose of this community service activity is to provide counseling on how to making and apply organic fertilizers, especially for vegetable crops. The counseling participants came from the Ponorogo Organic Farming Community in Jabung Village, Ponorogo Regency. Outreach to farmers using lectures, presentations, video screenings, and interactive discussions. Farmers' understanding increased by 37.5% on how to make and apply organic fertilizers. On average, farmers have an initial understanding level of 30%. After receiving counseling, the farmers' understanding increased to 67.5%.