Objectives: To evaluate the antibiofilm potential of Terminalia arjuna and Ipomea carnea plant extract against potent biofilm forming UTI pathogens. Methods/Statistical analysis:In this study, previously isolated and characterized three UTI pathogens viz. Escherichia coli, Pseudomonas aeruginosa and Staphylococcus equorum were used to evaluate antibiofilm potential of selected plants. Soxhlet apparatus was used with a solvent methanol for the extraction process. For the evaluation of antibiofilm activity of different concentrations (2, 4, 6, 8 and 10 mg/ml) of plant extract in 3% DMSO Crystal violet assay was used. Findings:The methanolic bark extract of T. arjuna showed maximum activity up to 89.84% at 10mg/ml. against S. equorum while leaves extract of I. carnea gives maximum antibiofilm activity up to 67.53% at 2mg/ml against E. coli. Applications: The result shows that the investigated plants might be helpful in the development of potent herbal drugs to treat UTI. https://www.indjst.org/ 2452 Urinary tract infections (UTIs) are the most common human infectious disease affecting the bladder, kidneys and urinary tracts. Escherichia coli is the most frequent pathogen causing UTI in humans and one of the most common causes of Gram-negative bacteremia in hospitalized patients Soxhlet extraction; Crystal violet assay