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Three isolates of Curvularia were obtained from diseased Eucalyptus sp., Syngonium vellozianum and Zinnia elegans. Phylogenetic trees inferred from combined dataset of the internal transcribed spacer ribosomal DNA (ITS), partial glyceraldehyde-3-phosphate dehydrogenase (GPDH), and partial translation elongation factor 1-α (EF1α) along with morphological characterization, showed they are two novel species. Curvularia ahvazensis sp. nov. and C. rouhanii sp. nov. are described and illustrated and shown to be morphologically distinct from previously known species of Curvularia.