Cytoplasmic male sterility (CMS) is a cornerstone of hybrid production in many crops. In three-line hybrid systems, use of CMS, maintainer, and fertility restorer lines is necessary for production of hybrid seeds. Limited resources of CMS and low variation of CMS lines cause genetic vulnerability to pathogens. Therefore, diversifying the CMS sources is indispensible for a sustainable production system of hybrid seed. In this study, we attempted for the first time to transfer CMS into maintainer line Yosen B in restricted generations using the marker-assisted backcrossing (MABC) method. The resultant F 1 hybrid of IR68897 A/Yosen B cross was backcrossed to Yosen B, and CMS plants in each backcross generation (from BC 1 F 1 to BC 3 F 1 ) were selected based on phenotyping test and MABC. Molecular assessment of backcross progenies was conducted using a mitochondrial CMS-specific marker and 34 polymorphic nuclear simple-sequence repeat (SSR) markers in early generations (from BC 1 F 1 to BC 2 F 1 ) and was continued using 9 additional SSRs and 82 inter-simple sequence repeat (ISSR) markers in BC 3 F 1 . A MABC strategy could successfully recover the recurrent parent genome (RPG) in BC 3 F 1 generation, and decreased heterozygosity of final CMS plants. Restorability test with known wild-abortive restorer lines (viz. IR36 and IR24) showed that combination of Yosen A  IR24 could produce highly fertile F 1 hybrid. Evaluation of some important agronomic traits of the final CMS line (BC 4 F 1 ) at field condition showed that it was comparable to the original maintainer fertile counterpart. Phenotypic and marker-assisted selections could considerably decrease the time needed for full recovery of RPG so that final CMS line could show a high similarity to original fertile counterpart.

A study was conducted on heterosis, combining ability and genetic parameters of yield and yield components in rice. Five lines were crossed with two testers in line × tester manner to produce ten F 1 hybrids. Results show that general combining ability (GCA) effect was only significant for total number of kernels per panicle, number of filled kernels and grain yield per plant, and specific combining ability (SCA) effect was significant for yield and all of its studied components (except for 100-kernel weight). Lines IR42 and Pouya showed a significant GCA for grain yield in opposite direction (20.9 and-13.7 g/plant, respectively). The two lines also showed highest significant GCA for number of filled kernels (22.7 and 23.3, respectively). In the total number of kernels, lines IR8 and IR42 and tester Usen showed the highest significant GCA (34.79, 27.97 and 12.56). In tiller number, only line IR36 and tester IR68897 had the highest significant GCA (3.51 and 0.84). Combination of IR68897×IR8 showed highest significant SCA for grain yield (9.7 g/plant), while in the case of number of filled kernels and tiller number, combinations IR68897×IR8 and Usen/IR36 showed a significant positive SCA (18.9 and 2.1, respectively), indicating that hybridization can be a choice for improving hybrids with better quantity of these traits. The highest general heritability (2 b h) was obtained for tiller number (96.1%), indicating slight effects of the environment on the trait, while for other traits, a mild general heritability (~70%) was obtained, indicating considerable effect of environment on phenotypic expression of most yield traits. A low specific heritability (2 n h) was obtained for all traits (18.2 to 26.3%), indicating that non-additive effects play an important role in genetic control of yield traits. Therefore, it seems that hybridization must be a choice for utilizing the putative heterosis in special crosses, and such a condition was observed for tiller number and grain yield in combinations of IR42×IR68897 and IR42×Usen.