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A study was conducted to determine the effect of supplementing the diet of breeder hens with a shortchain fatty acid (SCFA) premix, containing 509 g fatty acid salts/kg of which 285 g were calcium butyrate, on their eggshell characteristics and the hatching percentage of the eggs. One thousand six hundred 66-week old White Bovans laying breeder hens were used in this experiment. They were housed in eight identical pens, each containing 200 birds, and four pens were used per treatment. The SCFA premix was included at 1000 mg/kg in the treatment diet, and fed for a period of nine weeks. Responses were compared with an unsupplemented treatment. Supplementation started when the hens were 66 weeks old. From day 75 eggs were collected for the next seven weeks and the occurrence of cracked, dirty and misshapen eggs was recorded, and the hatching percentage of the eggs was determined. Eggshell strength was lower in eggs from the control (1.76 ± 0.05) than from the treatment group (2.07 ± 0.03). The percentage of eggs produced by the control group (68.6 ± 0.08) was significantly lower than that by the supplemented group (71.5 ± 0.15). Percentage of dirty, cracked and misshapen eggs, and the hatchability percentage of the control group (1.15 ± 0.03, 3.44 ± 0.05, 6.27 ± 0.03 and 88.93 ± 0.06, respectively) were also significantly lower than in the group receiving SCFA (0.47 ± 0.03, 2.21 ± 0.03, 3.81 ± 0.03 and 93.36 ± 0.05, respectively). It was concluded that dietary supplementation of SCFA to layer breeder hens from 66 weeks of age onwards improved eggshell strength, reduced the percentage of dirty, cracked and misshapen eggs and increased the hatching percentage of the eggs. The positive responses were suggested to be largely due to the butyrate in the SCFA. _______________________________________________________________________________________

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The objective of this study was to compare some husbandry procedures on the base of physiological stress parameters and evaluate the welfare status in sheep. Forty ewes were used as the study material. Measurements were taken during several routine husbandry procedures such as milking, shearing, weighing, loading and hoof care. Data regarding time spent for each application, as well as heart and respiratory rates were recorded during the applications. Blood samples were taken 15 min before and after each application and malondialdehyde (MDA), glutathione-peroxidase (GSH-Px), cortisol T 3 and T 4 parameters were measured. In addition, changes in the same parameters between pre-and post-application periods were evaluated. According to the results, machine milking caused less stress than hand milking. No significant difference was seen between shearing methods for hand shearer or clipper; however, both applications caused stress in animals. The results for weighing methods of animals demonstrated significant differences in cortisol, T 3 and T 4 values in favor of traditional method. Cortisol, T 3 and T 4 levels were significantly higher in manual loading compared with loading by ramp. Regarding hoof care, all the examined parameters differed in favor of modern method. On the other hand, significant differences were determined between the stress parameters regarding pre-and postapplications. All values differed for hand milking while no significant difference was observed in MDA and T 3 values in machine milking group. Parameters in weighing groups changed significantly. For loading process, GSH, cortisol, T 3 and T 4 values differed in both treatment groups. With regard to hoof care, parameters except T 4 in laying group differed significantly. An increase occurred in minute-based measurements of heart and respiratory rates parallel to physiological data. The number of the respiratory rates during the applications differed except for the shearing process. All the parameters displayed significant differences between groups in terms of heart rates. Time spent for each application also differed between groups. Time saved for milking, shearing, weighing, loading and hoof care was 3.23 min, 4.37 min, 1.71 min, 7.85 s and 1.55 min, respectively. These results appear to provide a tangible advantage of using new husbandry methods to the breeders. It was concluded that using new methods in sheep husbandry procedures provided advantages in terms of saving time and reducing labor, as well as improved conditions for welfare of animals. In addition, it facilitated the routine works and flock husbandry.

In this study, we evaluated the efficacy, expressed as a mean weight decrease of the whole echinococcal cyst mass, of novel benzimidazole salt formulations in a murine Echinococcus granulosus infection model. BALB/c mice were intraperitoneally infected with protoscoleces of E. granulosus (genotype G1). At 9 months post-infection, treatment with albendazole (ABZ), ricobendazole (RBZ) salt formulations, and RBZ enantiomer salts (R)-(+)-RBZ-Na and (S)-(−)-RBZ-Na formulations were initiated. Drugs were orally applied by gavage at 10 mg kg−1 body weight per day during 30 days. Experimental treatments with benzimidazole sodium salts resulted in a significant reduction of the weight of cysts compared to conventional ABZ treatment, except for the (S)-(−)-RBZ-Na enantiomer formulation. Scanning electron microscopy and histological inspection revealed that treatments impacted not only the structural integrity of the parasite tissue in the germinal layer, but also induced alterations in the laminated layer. Overall, these results demonstrate the improved efficacy of benzimidazole salt formulations compared to conventional ABZ treatment in experimental murine cystic echinococcosis.