Objective: We investigated the importance of netrin-1 levels in Ulcerative Colitis (UC) in clinical activity of the disease, and its association with other proinflammatory cytokines IL-6 and TNF-alpha.Materials and Methods: This study was a case-control study and included 67 patients with UC (36 activated, 31 in remission) and 50 healthy controls. UC patients were divided into mild activation (n=21), moderate activation (n= 6) and severe activation (n= 9) groups according to the "Truelove Witts clinical activity index". 31 asymptomatic patients were considered to be in remission. Netrin-1, IL-6 and TNF-alpha measurements in plasma samples were made using ELISA assay kit.Results: Between the patient group and the control group; there was a statistically significant difference between netrin-1, IL-6, TNF-alpha, (p<0.05 for all). The plasma netrin-1 mean of UC with severe activation groupwas statistically significantly higher than that of the mild activation, remission group and control group (p<0.05). Plasma netrin-1 mean of UC with modarate activation group was statistically significantly higher than that of the mild activation and remission group.Conclusion: We found that plasma netrin-1 levels increase with disease severity in UC, similar to proinflammatory cytokines such as TNF-alpha and IL-6.