Aim: This study was aimed to determine the efficacy of local bupivacaine injection into the incision site before diagnostic laparoscopy to reduce postoperative pain among infertile patients. Materials and methods: This was a randomized controlled study on 168 consecutive infertile women who underwent diagnostic laparoscopy at Farida Clinic and Infertility Management Center, Dhaka, from February to November 2018. Twenty-two patients were excluded from the study due to not meeting the criteria and 144 patients were finally taken for randomization. Results: In this study, majority of patients (n = 79) belonged to age 21-30 years. The mean age was found to be 29.7 ± 4.5 years in group I and 30.2 ± 4.7 years in group II. Majority of the patients had primary infertility in both groups, which was 47 (65.3%) in group I and 57 (79.2%) in group II. The commonest comorbidity was hypothyroidism found in both groups, 9 (12.5%) in group I and 7 (9.7%) in group II. Nine (12.5%) patients had previous operation in group I and 13 (18.1%) in group II. This study showed that majority of patients had mild pain 1 hour after surgery-48 (66.7%) in group I and 50 (69.4%) in group II. Three hours after surgery majority of patients in both groups had no pain 43 (59.7%) in group I and 40 (55.6%) in group II. Twelve hours after surgery, there was no pain in 64 patients (88.9%) in group I and 65 patients (90.3%) in group II. After 7 days of surgery, postoperative pain was absent in 45 (62.5%) patients in group I and 42 (58.3%) patients in group II, respectively. Conclusion:Considering the result of the study, at 1, 3, 12 hours and 7 days after surgery, pain score was not statistically significant (p > 0.05) between intervention and nonintervention groups. So, port-site infiltration of bupivacaine does not reduce the postoperative pain following laparoscopy.