Background: Diagnostic questionnaires play a great role in accelerating the diagnosis of mental disorders. Objectives: This study aimed to provide a cross-cultural adaption form of Self-report oppositional defiant behavior inventory (SR-ODBI) in Persian and assess the validity and reliability of this Persian form. Methods: The present study was done on two research samples, including a sample of 294 students who were selected in the school year of 2019 - 2020 (girls and boys) from high schools of Dezful city by multi-stage random sampling method and a sample of 320 parents. The validity of the oppositional defiant behavior inventory was assessed by two methods of confirmatory factor analysis and convergent validity, and the reliability of the inventory was assessed using Cronbach's alpha and split-half methods. Results: Cronbach's alpha was obtained at 0.73 (0.87) for the whole self-report scale (parent version), 0.72 (0.74) for the subscale of irritability, and 0.81 (0.80) for the subscale of stubborn and resentful behavior. The correlation between SR-ODBI and Achenbach Youth Mental Health Test was 0.56 (P < 0.01). The results of confirmatory factor analysis (RMSEA = 0.06 and 0.08) also indicated a relatively good fit of structures of the oppositional defiant behavior inventory. Conclusions: The results of the research indicated that the Persian version of the Oppositional Defiant Behavior Inventory in Iran has good reliability and validity.