Objective: The COVID-19 pandemic is still showing fluctuating trends and rapidly increasing case numbers, naturally creating a significant air of panic and hopelessness. This study aimed to investigate the mental health status of doctors in comparison to non-doctors, and its suggestive determinants amidst the COVID-19 pandemic. Methods: An online cross-sectional survey was conducted during January–March 2021, using a convenience sampling technique. A sample size of 377 was calculated through RaoSoft (RaoSoft Inc., Seattle, Washington, USA) software. Inventories used are Zung Self-Assessment Anxiety Score (SAS) and Self-Assessment Depression Score (SDS), for the purpose of comparing different groups. The survey was distributed as an online Google form via social media. Results were evaluated by IBM Statistical Package for Social Sciences version 26.0 (Endicott, New York, USA). Results: A total of 395 participants responded, out of which 10% showed extreme levels of anxiety. Among doctors, 14% had moderate levels of anxiety while 10% of unmarried respondents had extreme self-reported anxiety. Out of non-doctors, 15% showed signs of depression, among which a moderate level of depression was seen in 13% males, and severe depression was seen in 4% of married respondents. Higher depression scores were recorded in non-doctors, while there was no significant difference in anxiety levels of both groups. Conclusion: A significant impact on mental health has been noted in various groups of respondents, with severe depression as well as severe anxiety. This should be alarming enough to instigate authorities to conduct mental health programs to counter this negative impact of COVID-19. Quick interventions and strategies can save countries from a grim future.

Background: Tuberculosis (TB) is major health issue across the world. Yearly 2 million deaths are reported from this disease. Pakistan has been ranked in the top six countries that are bearing the major brunt of TB. Aim: To observe hepatoprotective role of aqueous extract of kasni (Chichorium intybus) roots on hepatotoxicity caused by Pyrazinamide in male BALB/c mice. Study Design: Randomized control Trial. Methodology: Healthy male albino Balb/C mice, fifty six in number were selected randomly and further grouped into four groups (n=14 mice). Group A labelled as control group and maintained on rodent diet with no medication. Group B was labelled as disease control group and only pyrazinamide was administered to mice in this group in dose (500mg/kg BW). Groups C and D designated as exploratory groups. Both group C & D were administered a combination of Anti TB drug, pyrazinamide along with aqueous extract preparations of Kasni roots in low (200mg/kg) & high doses (400mg/kg) respectively. Baseline blood samples were drawn at day zero. Results: Serum alanine aminotransferase (ALT) and alkaline Phosphatase (ALP) were improved in group C & D given aqueous extract of Chicorium intybus roots. Conclusion: It was concluded that high doses of aqueous extract preparations of kasni (Chichorium intybus) roots showed more improvements of serum markers in drug induced hepatotoxicity than low doses of aqueous preparations. Keywords: Chichorium intybus, Hepatoprotective, Alanine aminotransferase (ALT), Alkaline Phosphatase (ALP),

Background: Nephrotoxicity is characterized by raised serum urea and creatinine levels and kidneys being one of the primary organs of drug concentration and excretion are vulnerable to many widely used marketed drugs, including anti-cancer drugs, antibiotics, immunosuppressants, and radio contrast agents, are nephrotoxic. Aim: To determine the nephroprotective effect of ethanolic extract of Carica papaya seeds in Aminoglycoside induced acute nephrotoxicity. Study design: Quasi Experimental study. Methodology: Thirty Sprague Dawley rats were sub divided into 3 groups i.e., I, II and III with 10 rats in each group. Group II and III were administered Aminoglycoside drug; Gentamycin in 80 mg/kg) via intraperitoneal route once daily for 5 consecutive days to induce acute nephrotoxicity. At day 6, nephrotoxicity was confirmed by measuring serum urea and creatinine. Ethanolic extract of Carica papaya seeds (1000 mg/kg) was started once daily through oral route in group III for 5 consecutive days to see the nephroprotective effects of seed extract after causing acute kidney injury. All animals were given standard diet pellets manufactured at NIH. Results: Mean serum urea and creatinine for Group I (Control Group) at day 0 were 24.90 mg/dL ± 1.633 and .750 mg/dL ± .0619 respectively. Mean serum urea and creatinine for Group II (Disease Control Group) was 81.00 mg/dL ± 1.247 and 1.980mg/dL ± .0467 at day 6th. This suggested induction of nephrotoxicity by Gentamycin. Mean serum urea and creatinine for Group III (Ethanolic Extract Treated Group) at 11 day was 72.40mg/dL± .991 and 1.680 mg/dL± .0467 after 5 days treatment with ethanolic extract of Carica papaya seeds. Conclusion: We concluded that ethanolic extract of Carica papaya seeds has significant nephroprotective effects on Aminoglycoside induced acute nephrotoxicity in rats. Keywords: COVID-19, Vaccination, Awareness and Adverse Effects

Background: Kidneys are susceptible to many commonly used drugs, owing to their property of filtering, reabsorbing, and excreting multiple drugs. Aim: To study the renoprotective effect of ethanolic seed extract of Carica papaya on renal histomorphology in nephrotoxicity caused by Gentamicin. Study design: Experimental study. Methodology: Rats (n=30) were subdivided into 3 equal groups. Group I (control group) and Group II & III were administered Gentamicin (80 mg/kg) was given daily for 05 days continuously, intra-peritoneally to cause nephro-toxicity. Histomorphological examination of kidney tissue was done on day-6. In group-III, ethanolic extract of Carica papaya seeds (1000 mg/kg) was given daily for 5 days orally for its induction of nephro-protective effects. Data was entered and analyzed in computer using SPSS. Results: Acute Tubular Necrosis (ATN) and presence of Hyaline casts in the lumen of proximal tubules were the distinctive morphological characteristics. Histopathological specimens of the kidney in Ethanolic treated group showed moderate characteristics of renal damage as compared to that of the disease control group. Conclusion: Study concluded that that Carica papaya seed extract can significantly ameliorate the nephrotoxic effects of Gentamicin thus nephro-protective. Keywords: Gentamicin, Carica papaya, Nephrotoxicity and Histomorphology.