Premenopause is a transitional period that occurs due to decreased generative or endocrine function of the ovaries. In premenopausal women, there is a decrease in estrogen hormone levels and an increase in Body Mass Index (BMI) so that it will increase the risk of diabetes mellitus which is one of the four priority non-communicable diseases. The incidence of diabetes mellitus in women is much higher when compared to men. The research was conducted in the city of Padang, with an observational, cross-sectional design. The sampling technique used was multistage random sampling with a sample of 99 premenopausal women. The estradiol level was assessed by ELISA method, BMI was assessed by weight (kg) divided by height (m) squared, fasting blood glucose level was assessed by the enzymatic photometric method. The correlation between estradiol levels and fasting blood glucose levels was analyzed by using the Pearson Correlation test. The correlation between BMI and fasting blood glucose levels was analyzed by using the Pearson Correlation test. The mean of estradiol level was (148,689±49,417) pg/ml. Average Body Mass Index (BMI) was included in the Obese category I (26.5 kg/m2). The mean fasting blood glucose level was (98,766±6,549) mg/dL. There is a correlation between estradiol levels and fasting blood glucose levels (p value = 0.007 and r value = 0.270). In addition, there is a correlation between BMI and fasting blood glucose levels (p value = 0.023). The conclusion of this study is that there is a significant correlation between estradiol levels and fasting blood glucose. There is a significant correlation between BMI and fasting blood glucose Keywords: Estradiol Levels, Body Mass Index, Fasting Blood Glucose Levels, Premenopause.