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Mushroom tissue was immobilized onto plain screen printed electrode (SPE) and multiwalled carbon nanotube (MWCNT) modified SPE (SPE/MWCNT) via bismuth (Bi) deposition. By this way, SPE/Bi/Tissue and SPE/ MWCNT/Bi/Tissue biosensors for phenol detection were obtained. UV-visible spectroscopy and SEM were utilized for explain the immobilization procedure. After optimizing working parameters, both biosensors were compared in terms of analytical characteristics. Linear range of response were found as 5 -100 mM for SPE/Bi/Tissue and 2 -200 mM for SPE/MWCNT/Bi/Tissue while detection limits were calculated as 0.48 mM and 1.17 mM for SPE/Bi/ Tissue and SPE/MWCNT/Bi/Tissue respectively. Developed systems were also applied for phenol detection in synthetically prepared waste water sample and very promising results were obtained.