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Objectives: The use of nonsteroidal anti-inflammatory drugs (NSAIDs) after regenerative surgeries may affect the behavior of stem cells used during the treatment. This study investigates the possible effect of NSAIDs on the proliferation and the osteo-differentiation of dental pulp stem cells (DPSCs) in vitro. Methods : DPSCs were isolated, cultured and characterized with human antibodies CD90, CD105 and CD45. Cells were cultured for a period of 1, 3 and 5 days to assess the viability using different drug concentrations of acetylsalicylic acid (ASA) 10, 50, and 200 μg/mL., Diclofenac sodium 10 -4 and 10 -6 M and Meloxicam 0.01, 0.1, and 1 μM .Selected concentration of ASA 10 μg/ mL, Diclofenac sodium 10 -6 M and Meloxicam 0.1 μM were used during osteo-differentiation of the cells.Results : DPSCs viability assay recorded the highest absorption rate on the 1 st day with ASA 10 μg/mL and on the 3 rd day with Meloxicam 0.01 μM. On day 5 all concentration of ASA and Diclofenac reported the highest absorption rats. The highest values of calcium compounds was expressed with ASA 10 μg/mL on the 7 th day using alizarin red satin. Conclusion :Using different types of NSAIDs during the postsurgical regimen can affect the viability and osteo-differentiation of DPSCs.

Purpose: Diabetes mellitus (DM) has deleterious effect on various body organs especially salivary glands. Therefore, this study was conducted to evaluate the therapeutic effect of curcumin on parotid salivary glands in diabetic rats. Material and methods: Fifty-six adult male albino rats weighting 150-200 g were selected and divided into two equal groups n= 28 rats each. Diabetes was induced in both groups I (Diabetic) & II (Diabetic & curcumin) with a single intraperitoneal injection of streptozotocin (STZ) at a dose of 55 mg/kg. After 2 weeks of diabetes confirmation; rats of group II received 200 mg\kg of curcumin orally once daily. Both groups I and II were divided into 2 subgroups (A, B, C and D respectively) 14 rats each and were sacrificed after 15 and 20 days of curcumin administration, and the parotid glands sections were stained with Hematoxylin and Eosin, Anti-iNOS, and Anti-IL-2. Then were subjected to digital image analysis followed by two-way ANOVA statistical analysis. Results: Curcumin treated group revealed improvement in parotid glands in H&E sections especially at 20 days when compared to diabetic group with reduced histomorphological alterations. While, immunohistochemically, curcumin treated group showed decreased expression in the levels of both iNOS and IL-2 with significant statistical difference with diabetic group in all time periods. Post hoc LSD test showed a significant difference between time periods of diabetic group while showed no significance between curcumin group. Conclusion: Curcumin can be possible candidate for hindering the regressive changes that affect salivary glands caused by diabetes.

Purpose: This study was carried out in order to assess the effect of 10% PRF exudate obtained from controlled type 2 diabetic patients on proliferation capacity of dental pulp stem cells (DPSCs). Then 1% PRF exudate obtained from controlled type 2 diabetic patients was tested for osteogenic capability after 1 week. Material and methods: PRF exudates were prepared using the direct method from 8 healthy donors (control group) and 8 controlled type 2 diabetic patients (study group). In 96-well plates, DPSCs harvested from pulp tissue of impacted lower third molar were treated with 10% PRF exudate obtained from healthy donors and controlled type 2 diabetic patients for 1,3 and 5 days. The effects of exudates on proliferation and cell viability were tested using the MTS assay. For osteogenic capability, DPSCs were treated with 1%PRF exudate obtained from healthy donors and controlled type 2 diabetic patients supplemented with osteogenic medium for 1week and the osteodifferentiation assay was held using alizarin red stain with ELIZA reader. All data were subjected into one-way Anova statistical analysis. Results: The concentrations of 10% and 1% PRF exudates obtained from controlled type 2 diabetic patients had proliferative and osteoinductive effects on DPSCs comparable to that of the healthy individuals. Conclusion: Based on our study, controlled type 2 diabetic patients can be treated similar to their Unaffected peers using 10% PRF exudates as the optimal concentration for DPSCs proliferation and 1% PRF exudates as the optimal concentration for DPSCs osteodifferentiation and formation of mineralized nodules in 7 days.