Fluorescent carbon dots derived from peanut shells were prepared via a pyrolysis approach and used for living cell imaging.

The ratiometric fluorescence assay, which can eliminate the external effects, has attracted great attention. In this work, a carbon dot (CD)-based nanohybrid dual-emission system was simply prepared by a unique approach of solvothermal treating corn bract and used as a ratiometric fluorescent sensor for Hg detection. Under a single excitation, the obtained nanohybrid sensor had two emission bands around 470 and 678 nm, which may originate from the intrinsic structure of CDs and chlorophyll-derived porphyrins, respectively. In the presence of Hg, the fluorescence at 678 nm could be remarkably quenched, while the fluorescence intensity at 470 nm was only slightly altered. The fluorescence intensity ratio at 470 and 678 nm exhibited a good linear relationship in the Hg concentration range from 0 to 40 μM with a detection limit of about 9.0 nM. It also had a satisfying assay performance in serum and river water samples. The prepared CD-based nanohybrid sensor here may hold the further potential applications in biomedicine study, environmental protection, and food safety.

A green approach was developed for the preparation of fluorescent carbon dots (CDs) by using lychee seed as precursor. The preparation of CDs was performed by simply pyrolysis. The quantum yield of asprepared CDs was 10.6% by using quinine sulfate as the reference. The CDs were employed as a fluorescence probe for the detection of methylene blue (MB). This sensing system exhibits excellent 10 sensitivity and selectivity toward MB, and a detection limit of 50 mM is achieved. The possible application of as-prepared CDs for imaging in living cells was also explored. The inherent cytotoxicity of CDs was evaluated using HepG2 cell, and the cell viabilities were estimated to be greater than 90% upon addition of the CDs over a wide concentration range of 0−1000 µg/mL. It was then successfully applied for the fluorescence imaging of HepG2 cells. 15 2 Experimental Materials and reagentsFresh lychee was purchased from a local market. After its skin 75

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