A templating strategy using crosslinked and functionalized polymeric beads to synthesize silica microspheres with a broad pore size distribution has been developed. The polymer/silica hybrid microspheres were prepared by utilizing the combination of a templating weak cation exchange resin, a structure-directing agent N-trimethoxysilylpropyl-N,N,N-trimethylammonium chloride, and a silica precursor tetraethyl orthosilicate. The silica microspheres were then obtained after calcinating the hybrid microspheres. The as-prepared materials were characterized by scanning electron microscopy, mercury intrusion porosimeter, and thermal gravimetric analysis. The results showed that the starting templating beads were about 5 μm in diameter and the formed silica microspheres were less than 3 μm with a pore size range of 10-150 nm, some pores were even extended to beyond 250 nm. It was demonstrated that cellulose tris(3,5-dimethylphenylcarbamate) was readily coated onto the surface of the as-synthesized silica microspheres without any additional surface pretreatment. The coated silica microspheres were uniformly dispersed even with high loading of the chiral stationary phase, which exhibited high resolution chiral separations in high-performance liquid chromatography.

Large-pore silica microspheres were synthesized by utilizing weak cation exchange polymer beads as templates, -trimethoxysilylpropyl--trimethylammonium chloride (TMSPTMA) as a structure-directing agent, tetraethoxysilane (TEOS) as a silica precursor, and triethanolamine as a weak base catalyst. The hydrolysis and condensation of the silica precursors occurred inside the templating polymer beads yielded polymer/silica composite microspheres. After the organic polymer templates were removed in the calcination step, large-pore silica microspheres were produced. The effects of different reaction conditions on the morphology, structure and dispersibility of the formed silica microspheres were investigated. It has been shown that when the volume ratio of TMSPTMA, TEOS and triethanolamine was 1:2:2, silica microspheres with pore size range of 50-150 nm and particle size around 2 μm were obtained. The as-prepared silica microspheres were then bonded with chlorodimethyloctadecylsilane (C18), packed into a 50 mm×4.6 mm column, and evaluated for the separations of some common standard proteins and soybean isolation proteins. The results showed that the large-pore silica spheres from this work have potentials for protein separation in HPLC.

The new economic model and the development of the Internet and media technology have bred the network mixed clip video. This new video type not only enriches people’s spiritual and cultural life, but also brings about copyright infringement problems. This article focuses on the copyright issue of Network remixes short video, and also focuses on the issues of whether the Network remixes short video belong to the “Works” stipulated in the Copyright Law of The People’s Republic of China and whether the use of other people’s works in the Network remixes short video can be regarded as reasonable, and hopes to put forward some suggestions for the prevention, solution or regulation of related problems.