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Background: Although it is recognized that dynamic stereopsis is vital in daily life, there is still room for improvement in assessment methods. A novel clinical dynamic stereopsis assessment method was created based on an autostereoscopic display system that did not require additional auxiliary glasses. This study evaluated the optical parameters and clinical performance of the autostereoscopic display system for clinical dynamic stereopsis assessment. Methods:The autostereoscopic dynamic stereopsis assessment device was based on a directional backlight technology. Experiment 1 was performed under the same environmental conditions to compare luminance, crosstalk, and spectrum between the autostereoscopic dynamic stereopsis assessment device and the conventional dynamic random-dot stereopsis measuring instrument. Experiment 2 was an observational, analytic, cross-sectional study involving 135 healthy participants, each of whom was asked to complete measurements on both the autostereoscopic and conventional devices in random order. Stereo acuity, operating time, acceptance, and visual fatigue scores were recorded for clinical evaluation. Results:The autostereoscopic device had brighter luminance (139 and 140 cd/m 2 for 2 eyes, respectively), lower crosstalk (4.50% for both eyes), and higher color restoration degree than those of the conventional instrument. Clinically, the novel dynamic stereopsis assessment was as accurate as the traditional method [170" (0.00") and 170" (0.00") respectively; P=0.317], and with more efficiency (166±58.9 and 298±116 s, respectively; P<0.001), higher acceptance (3.36±0.93 and 2.02±0.59 points, respectively; P<0.001), lesser fatigue (0.27±0.46 and 0.73±0.66 points, respectively; P<0.001). The autostereoscopic dynamic stereopsis assessment device with brighter luminance, lower crosstalk, and higher color restoration degree was more effective than the traditional instrument at displaying dynamic clues for clinical dynamic stereopsis assessment. Furthermore, its high-quality image and user-friendly interface provided accurate assessment results in all 3 dynamic stereopsis assessment task conditions, with a higher level of acceptance and lesser visual fatigue, than the traditional assessment method. Conclusions:The autostereoscopic device has excellent functions in both optical parameters and clinical performance, and therefore has the potential to be applied and popularized in future assessments.

The perceptual experience issue is particularly important for autostereoscopic three‐dimensional (3D) displays since the perceptual resolution has a great correlation between panel resolution and other 3D display performances, but normally used effective resolution only considers the amount of information being sent to a certain viewing point. This work describes that the visual factors should be taken into consideration associated with resolution and contrast simultaneously to evaluate perceptual 3D resolution. We propose a quantitative evaluation of perceptual resolution of 3D displays from modulated contrast based on the contrast sensitivity function (CSF). A switchable parallax‐barrier display and a directional backlight display providing glasses‐free autostereoscopic vision are experimentally studied to verify the validity of the characterization. Due to the nonlinear response of the CSF threshold curve, less than 50% of the panel resolution in parallax‐barrier 3D displays is perceived, while the display with directional backlight illumination is shown to retain the panel resolution for perceptual experience as expected. We foresee that this study provides a practically objective paradigm to characterize the subjective perceptual resolution and the presented perceptual resolution can be served as a design guideline to achieve an improved 3D viewing experience.

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