Modern agriculture systems are copiously dependent on agrochemicals such as chemical fertilizers and pesticides intended to increase crop production and yield. The indiscriminate use of these chemicals not only affects the growth of plants due to the accumulation of toxic compounds, but also degrades the quality and life-supporting properties of soil. There is a dire need to develop some green approach that can resolve these issues and restore soil fertility and sustainability. The use of plant biostimulants has emerged as an environmentally friendly and acceptable method to increase crop productivity. Biostimulants contain biological substances which may be capable of increasing or stimulating plant growth in an eco-friendly manner. They are mostly biofertilizers that provide nutrients and protect plants from environmental stresses such as drought and salinity. In contrast to the protection of crop products, biostimulants not only act on the plant’s vigor but also do not respond to direct actions against pests or diseases. Plant biostimulants improve nutrient mobilization and uptake, tolerance to stress, and thus crop quality when applied to plants directly or in the rhizospheric region. They foster plant growth and development by positively affecting the crop life-cycle starting from seed germination to plant maturity. Legalized application of biostimulants causes no hazardous effects on the environment and primarily provides nutrition to plants. It nurtures the growth of soil microorganisms, which leads to enhanced soil fertility and also improves plant metabolism. Additionally, it may positively influence the exogenous microbes and alter the equilibrium of the microfloral composition of the soil milieu. This review frequently cites the characterization of microbial plant biostimulants that belong to either a high-risk group or are closely related to human pathogens such as Pueudomonas, Klebsiella, Enterobacter, Acinetobacter, etc. These related pathogens cause ailments including septicemia, gastroenteritis, wound infections, inflammation in the respiratory system, meningitis, etc., of varied severity under different conditions of health status such as immunocompromized and comorbidity. Thus it may attract the related concern to review the risk status of biostimulants for their legalized applications in agriculture. This study mainly emphasizes microbial plant biostimulants and their safe application concerns.

Soil microbial community structure is influenced by both biotic and abiotic factors prevailing in the soil milieu. The pH is one of the noticeable abiotic factors that affect soil microbial community. Different species prefer different range of pH for their optimal growth; however they can tolerate a wide range around acidic, neutral or alkaline pH. Microbes with broad range of pH tolerance i.e. from acidic to alkaline soil have better survival rate or opportunity as compared to other microbes which have narrow range of pH tolerance. So, in the present study growth response of plant growth promoting bacteria (PGPB) from rhizosphere and rhizoplane of different groups of plant has been characterized at broad range of pH (3.0-13.0) and hence can be applied in soils of different pH range to enhance plant growth and yield. Ten bacterial isolates, which exhibited in-vitro plant growth promoting traits i.e. phosphate solubilisation, biological nitrogen fixation, Indole acetic acid (IAA) production and siderophore production, revealed differential growth response to pH. Some isolates showed good response in broad pH range from acidic to alkaline (~3.5 to ~12.5). These plant growth promoting bacterial groups therefore can be potentially used as bacterial consortium for application in agricultural fields to enhance crop productivity.

This study aimed to characterize, validate, and evaluate the plant growth potential of bacterial isolates (E-2, T-2, and T-1) to determine their suitability for application as biofertilizers and/or plant-biostimulants. The plant growth-promoting potential of bacteria (E-2, T-2, and T-1) has been validated in a hydroponic study on paddy plants by inoculating bacterial isolates and monitoring the phenotypic and plant growth responses. The applicability of bacteria was tested based on their tolerance to salinity, susceptibility to antibiotics, and identification based on 16S rDNA sequencing. The isolates E-2, T-2, and T-1 improved plant growth variably and significantly (P < 0.05 at 95% confidence interval) when inoculated into the plant growth matrix, ensuring nutrient availability to the plants grown under a nutrient (nitrate or phosphate) deprived growth matrix. Isolates E-2, T-2, and T-1 grew at salt (NaCl) concentrations of 7%, 6%, and 6%, respectively, and were tolerant to saline conditions. Although these three isolates exhibited resistance to certain antibiotics, they were susceptible to a large number of readily available antibiotics. Isolates E-2, T-2, and T-1 were identified as Klebsiella sp. strain BAB-6433, Citrobacter freundii strain R2A5, and Citrobacter sp. DY1981 respectively, and all of these may be assigned to Risk-Group-2 and hence are safe in view of their susceptibility to readily available antibiotics. Hence, these isolates are promising for extensive evaluation as bioinoculants to ecologically improve soil quality, fertility, crop growth, and yield.