A series of 4-iminonaringenin derivatives 2-6 have been prepared in good overall yields from a condensation reaction between naringenin and primary amines. The structures of all products were confirmed by ultraviolet, infrared, proton nuclear magnetic resonance, and carbon-13 nuclear magnetic resonance spectroscopic techniques. These derivatives were analyzed by high-performance liquid chromatography using polysaccharide-based chiral stationary phases, namely, Chiralpak IB and Chiralcel OD, using various mobile phases. 2-Propanol showed a high enantioselectivity for naringin and its derivatives using achiral column containing immobilized polysaccharides (Chiralpak IB).

In this work the stability of Loperamide® hydrochloride has been described in different pH solutions with different buffer species. High performance liquid chromatography (HPLC) is an efficiency method of Loperamide® hydrochloride, but the direct analysis procedure is not suitable for the determination of Loperamide® hydrochloride in the presence of its acid degradation product or its impurity. The present work aims to develop and validate a new feasible, sensitive and specific analytical procedure using HPLC on chiral columns.