The dichloromethane extract of the roots of Prangos hulusii, a recently described endemic species from Turkey, has yielded nine known and one new prenylated coumarins. The structures were elucidated by spectroscopic methods and direct comparison with the reference compounds where available. The root extract and its prenylated coumarins exhibit antimicrobial activity against nine standard and six clinically isolated strains at a concentration between 5 and 125 µg/mL. In particular, the new coumarin, 4′-senecioiloxyosthol (1), displayed 5 µg/mL MIC (Minimum Inhibitory Concentration) value against Bacillus subtilis ATCC 9372, murraol (4) and auraptenol (5) showed 63 µg/mL MIC value against Klebsiella pneumoniae ATCC 4352 and Bacillus subtilis ATCC 9372, and isoimperatorin (9) exhibited 16 µg/mL MIC value.

AbstractUropathogenic Escherichia coli (UPEC) is the most prevalent pathogen causing urinary tract infections (UTIs). UPECs have various virulence factors such as adhesins, biofilm forming and toxin producing etc., to survive in urinary tract. Under certain circumstances probiotics are preferred for prevention and treatment of UTIs. In this study, we aimed to investigate the in-vitro effects of four different Lactobacillus spp. cell-free supernatants on growth and biofilm formation inhibition in clinically isolated UPEC strains. Growths of 50 UPEC strains were determined in 96-well microplate and measured in a spectrophotometer after four hours incubation at 37°C. Biofilm formation was detected by crystal violet staining method on three UPEC strains. Statistical analysis of growth and biofilm formation experiments were performed by Kruskal-Wallis and one-way ANOVA Tukey’s multiple-comparison tests, respectively. All tested cell-free supernatans of lactobacilli inhibited growths (p<0. 0001) and biofilm formation (p<0.05) of UPECs. All results were found to be statistically significant. As a conclusion, our findings supported previous studies which reported the high efficiency of these four Lactobacillus spp. in the prevention of UTIs.

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