Abstrak Penelitian ini dilakukan untuk mengetahui apakah bioavailabilitas formulasi ibuprofen suppositoria 125 mg yang diproduksi oleh PT 90, 89, dan 85,16% untuk C max 0 (control), 20 min; 40 min; 1; 1,5; 2; 2,5; 3; 4; 6; 8; and 10 were, respectively, 28.59(3.37) µg.h.mL -1 , 30.47(3.56) µg.h.mL -1 , 8.24(1.44) µg/mL, and 1.33(0.44)

AbstrakPemberian jus jeruk dengan feksofenadin telah ditemukan menurunkan bioavailabilitas feksofenadin sampai kurang dari 30% melalui hambatan organic-anion transporting polypeptide (OATP) 81.7 (9.7-193.8)%, tidak berubah (n=4) 96.4 (80.5-107.9)%, menurun (n=6) 31.9 (9.7-49.0)%, meningkat (n=3) 161.8 (134.6-193.8)%; klaritromisin : total (n=12) 91.4 (20.6-158.3)%, tidak berubah (n=5) 103.1 (80.9-123.0)%, menurun (n=4) 34.8 (20.6-64.3)%, meningkat (n=3) 147.2 (132.9-158.3 Abstract Concomitant administration of orange juice with fexofenadine has been found to decrease the bioavailability of fenofenadine to less than 30% via inhibition of organic-anion transporting polypeptide (OATP), a drug uptake transporter expressed in organs such as liver, kidney and intestine. Erythromycin and clarithromycin are substrates and inhibitors of CYP3A4, a drug metabolizing enzyme in the liver and enterocytes, and P-glycoprotein (P-gp), a drug efflux transporter expressed in the same organs as OATP. Since an extensive overlap exists between substrates and inhibitors of CYP3A4, P-gp and OATP transporters, we want to study the effect of coadministration of our local orange (Siam orange)juice on the bioavailability of the above antibacterials. We conducted two 2-way cross-over randomized studies, one study for each antibacterial (500 mg), crossed between administration with orange juice (200 ml) and with water, in 12-13 healthy subjects per study. The serum concentrations of the antibacterials were assayed by microbiological method. The mean (range) ratio of AUC 0-t with orange juice/with water were as follows : erythromycin : total (n=13) 81.7 (9.7-193.8)%, unchanged (n=4) 96.4 (80.5-107.9)%, decreased (n=6) 31.9 (9.7-49.0)%, increased (n=3) 161.8 (134.6-193.8)%; clarithromycin : total (n=12) 91.4 (20.6-158.3)%, unchanged (n=5) 103.1 (80.9-123.0)%, decreased (n=4) 34.8 (20.6-64.3)%, increased (n=3) 147.2 (132.9-158.3

Introduction. Accumulation of glycochenodeoxycholic acid (GCDC) in serum has a clinical significance as an inductor of pathological hepatocyte apoptosis, which impairs liver function. Inhibition of GCDC accumulation can be used as a marker in therapy. This study was aimed to quantify the serum level of GCDC in obstructive jaundice patients. Methodology. GCDC acid level in the serum was quantified using high performance liquid chromatography (HPLC) technique according to Muraca and Ghoos modified method. It was performed before and after decompression at day 7 and day 14. The sample was extracted with solid phase extraction (SPE) technique on SPE column. The results were analyzed using SPSS V 16.0 (P < 0.05) and quantified with standard curve on GCDC acid. Result. There were 21 cases with range of GCDC acid serum level before decompression was 90.9 (SD 205.5) μmol/L and day 7 after decompression decreased to 4.0 (SD 46.4) μmol/L and then increased to 11.3 (SD 21.9) μmol/L (P < 0.05). This method could separate GCDC acid on serum with good resolution, high precision and accuracy, and linear calibration curve on measured level range. Conclusion. HPLC can quantify GCDC acid serum on obstructive jaundice patients and can be used to support its pharmacokinetic study.