Background: Lauric acid (LA), a common constituent of coconut oil is used as food additives and supplements in various formulations. Despite various potential pharmacological properties, no scientific evidence on its dose-related toxicity and safety is available till date. Objective: The current study was conducted to evaluate acute oral toxicity of LA on normal rats. Methods: Study was conducted in accordance with the Organization for Economic Cooperation and Development guidelines (OECD 423) with slight modifications. LA was administered orally to female Sprague Dawley (SD) rats (n = 6/group) at a single dose of 300 and 2000 mg/kg body weight respectively, while normal control received vehicle only. Animals from all the three groups were monitored for any behavioural and toxicological changes and mortality for two weeks. Food and fluid consumption, body weight was monitored on daily basis. At the end (on day 15 th) of the experimental period, blood was collected for haematological and biochemical analysis. Further, all the animals were euthanized, and internal organs were harvested for histopathological investigation using four different stainings; haematoxylin and eosin, Masson trichrome, Periodic Acid Schiff and Picro Sirius Red for gross pathology through microscopical observation. Results: Study result showed no LA treatment-related mortality and morbidity at two different dosages. Daily food and water consumption, body weight, relative organ weight, haematological, and biochemical analysis were observed to be normal with no severe alterations to the internal tissues. Conclusion: Current finding suggests that single oral administration of LA, even up to 2000 mg/kg body weight did not exhibit any signs of toxicity in SD rats, thus, safe to be used on disease model in animals.

Background: Lauric acid (LA), a common constituent of coconut oil is used as food additives and supplements in various formulations. Despite various potential pharmacological properties, no scientific evidence on its dose-related toxicity and safety is available till date. Objective: The current study was conducted to evaluate acute oral toxicity of LA on normal rats. Methods: Study was conducted in accordance with the Organization for Economic Cooperation and Development guidelines (OECD 423) with slight modifications. LA was administered orally to female Sprague Dawley (SD) rats (n = 6/group) at a single dose of 300 and 2000 mg/kg body weight respectively, while normal control received vehicle only. Animals from all the three groups were monitored for any behavioural and toxicological changes and mortality for two weeks. Food and fluid consumption, body weight was monitored on daily basis. At the end (on day 15 th) of the experimental period, blood was collected for haematological and biochemical analysis. Further, all the animals were euthanized, and internal organs were harvested for histopathological investigation using four different stainings; haematoxylin and eosin, Masson trichrome, Periodic Acid Schiff and Picro Sirius Red for gross pathology through microscopical observation. Results: Study result showed no LA treatment-related mortality and morbidity at two different dosages. Daily food and water consumption, body weight, relative organ weight, haematological, and biochemical analysis were observed to be normal with no severe alterations to the internal tissues. Conclusion: Current finding suggests that single oral administration of LA, even up to 2000 mg/kg body weight did not exhibit any signs of toxicity in SD rats, thus, safe to be used on disease model in animals.

Background. Arjunolic acid (AA) is a potent phytochemical with wider pharmacological activities. Despite potential medicinal properties on various in vitro and in vivo studies, there is still a dearth of scientific data related to its safety profile and toxicological parameters. The current study aimed to investigate acute toxicity of AA in normal female Sprague Dawley (SD) rats. Methods. In this study, AA was administered orally at an individual dose of 300 mg/kg and 2000 mg/kg body weight to group 1 and 2 respectively, while group 3 served as normal control. All the animals were observed for two weeks to determine any behavioral and physical changes. On day fifteen, blood was collected for hematological and biochemical investigation, later animals from all the three groups were euthanized to harvest and store essential organs for histopathological analysis. Four different staining techniques; hematoxylin and eosin (H&E), Masson trichrome (MT), Periodic acid Schiff (PAS) and Oil O Red were used to investigate any alterations in different tissues through microscopical observation. Results. Result of the study showed no morbidity and mortality at two different dosage of AA treatment. Daily food & water intake, body weight, relative organ weight, hematological and biochemical parameters were detected to be normal with no severe alteration seen through microscopical investigation in the structure of harvested tissues. Our finding support safety profile of AA which was well tolerated at higher dose, thus, warrants in-detail study on subacute disease model.

Background. Arjunolic acid (AA) is a potent phytochemical with wider pharmacological activities. Despite potential medicinal properties on various in vitro and in vivo studies, there is still a dearth of scientific data related to its safety profile and toxicological parameters. The current study aimed to investigate acute toxicity of AA in normal female Sprague Dawley (SD) rats. Methods. In this study, AA was administered orally at an individual dose of 300 mg/kg and 2000 mg/kg body weight to group 1 and 2 respectively, while group 3 served as normal control. All the animals were observed for two weeks to determine any behavioral and physical changes. On day fifteen, blood was collected for hematological and biochemical investigation, later animals from all the three groups were euthanized to harvest and store essential organs for histopathological analysis. Four different staining techniques; hematoxylin and eosin (H&E), Masson trichrome (MT), Periodic acid Schiff (PAS) and Oil O Red were used to investigate any alterations in different tissues through microscopical observation. Results. Result of the study showed no morbidity and mortality at two different dosage of AA treatment. Daily food & water intake, body weight, relative organ weight, hematological and biochemical parameters were detected to be normal with no severe alteration seen through microscopical investigation in the structure of harvested tissues. Our finding support safety profile of AA which was well tolerated at higher dose, thus, warrants in-detail study on subacute disease model.