Galectin-3 (Gal-3) is a β-galactoside-binding lectin, which is important in inflammation, fibrosis and heart failure. The present study aimed to investigate the role and mechanism of Gal-3 in hypoxia-induced pulmonary arterial hypertension (PAH). Male C57BL/6J and Gal-3−/− mice were exposed to hypoxia, then the right ventricular systolic pressure (RVSP) and Fulton's index were measured, and Gal-3 mRNA and protein expression in the pulmonary arteries was analyzed by reverse transcription-quantitative polymerase chain reaction and western blotting. Compared with the control, hypoxia increased the mRNA and protein expression levels of Gal-3 in wild type murine pulmonary arteries. Gal-3 deletion reduced the hypoxia-induced upregulation of RVSP and Fulton's index. Furthermore, human pulmonary arterial endothelial cells (HPAECs) and human pulmonary arterial smooth muscle cells (HPASMCs) were stimulated by hypoxia in vitro, and Gal-3 expression was inhibited by small interfering RNA. The inflammatory response of HPAECs, and the proliferation and cell cycle distribution of HPASMCs was also analyzed. Gal-3 inhibition alleviated the hypoxia-induced inflammatory response in HPAECs, including tumor necrosis factor-α and interleukin-1 secretion, expression of intercellular adhesion molecule-1 and adhesion of THP-1 monocytes. Gal-3 inhibition also reduced hypoxia-induced proliferation of HPASMCs, partially by reducing cyclin D1 expression and increasing p27 expression. Furthermore, Gal-3 inhibition suppressed HPASMC switching from a ‘contractile’ to a ‘synthetic’ phenotype. In conclusion, Gal-3 serves a fundamental role in hypoxia-induced PAH, and inhibition of Gal-3 may represent a novel therapeutic target for the treatment of hypoxia-induced PAH.

Aryl ketones were synthesized via Pd-catalyzed decarboxylative coupling between O-methyl oximes and phenylglyoxylic acids using (NH(4))(2)S(2)O(8) as the oxidant.

Empirical findings from the impact of innovation investment volatility on enterprise technological innovation are mixed. Based on the punctuated equilibrium theory, this study explores the impact of innovation investment volatility on enterprise technological innovation in different life cycles and whether innovation subsidy has expected effects on enterprises’ technological innovation. By using the 205 Chinese listed enterprises in strategic emerging industries from 2010 to 2019 as the research sample, the results show that the innovation investment volatility has a positive impact on technological innovation of enterprise in the growing stage, while it has no significant effect on enterprise technological innovation in the mature and declining stages. In addition, the negative moderating effect of innovation subsidy on the relationship between innovation investment volatility and technological innovation is the most significant for enterprises in the growing stage, weakly significant for enterprises in the mature stage, and insignificant for enterprises in the declining stage.

PurposeThe purpose of this study is to explore how government subsidies influence technology innovation in new-energy enterprises in the new era of Industry 4.0. Specifically, this study investigates the mediating effect of digital transformation and the moderating effect of a top management team (TMT) with digital experience.Design/methodology/approachUsing a sample of 225 listed new-energy companies, with annual information, patent data, and financial data for the years 2010–2020, this study employs panel fixed effect regression models to obtain the results.FindingsThis study finds strong evidence that government subsidies promote the technology innovation of new-energy enterprises, and digital transformation partially mediates the effect of government subsidies on technology innovation. In addition, a TMT's digital experience moderates the effect of government subsidies on digital transformation, but has no significant moderating effect on the relationship between digital transformation and technology innovation. Further analysis shows that subsidies make a sustained contribution to both digital transformation and technological innovation over the next two years. The digital subsidies have a stronger role in promoting digital transformation and further technological innovation through digital transformation.Practical implicationsThe Chinese government needs to continue to intermittently increase subsidies for new-energy enterprises, and focus on guiding enterprises' digital transformation. Chinese new-energy enterprises should pay attention to the importance of having TMTs with digital experience, make full use of government subsidies, actively implement digital transformation, and improve their innovation levels.Originality/valueA new conceptual framework is proposed to examine the relationships between government subsidies, digital transformation, a TMT's digital experience, and technology innovation. This paper provides an important theoretical basis and practical reference for improving the technology innovation ability of Chinese new-energy enterprises, and the high-quality development of renewable energy in the context of Industry 4.0.