Objective To develop and demonstrate the feasibility of a method for estimating lifetime hip and knee cumulative joint force using survey data on physical activity, and to construct and describe lifetime trajectories of energy expenditure and hip and knee joint force. Methods Exposure data on lifetime physical activity, including type (occupational, household, and recreation) and dose (frequency, intensity, and duration), were collected from a Canada‐wide population study of adults ages ≥45 years. Subjects were ranked in 2 ways: in terms of physical activity–related energy expenditure and in terms of a cumulative peak force index (CPFI) for the hip and knee, which is a measure of lifetime exposure and is a time/joint force product involving years of force and subject bodyweight. A relative joint loading index was calculated as the ratio of joint force (CPFI score) to energy expenditure. Results A total of 4,269 subjects completed the baseline measurements. Lifetime energy expenditure and hip and knee CPFI scores were higher for occupational and household activity than sport. The mean lifetime energy expenditure from total physical activity in the study sample was 119.1 metabolic equivalent‐hours/week. Women had slightly higher total lifetime energy expenditure and CPFI scores than men. The relative joint loading index was highest for male household and sport activity and lowest for female occupational activity. Conclusion Lifetime cumulative hip/knee joint force trajectories were successfully constructed from survey data and followed expected trends. Comparing energy expenditure with joint force revealed variation by age, sex, and activity type, indicating these measures may help distinguish the numerous benefits of physical activity from possible risks.

Development of methods for rapid distinction between cancerous and non-neoplastic tissues is an important goal in disease diagnosis. To this end, desorption electrospray ionization mass spectrometry (DESI-MS) imaging was applied to analyze the lipid profiles of thin tissue sections of 68 samples of human prostate cancer and normal tissue. The disease state of the tissue sections was determined by independent histopathological examination. Cholesterol sulfate was identified as a differentiating compound, found almost exclusively in cancerous tissues including tissue containing precancerous lesions. The presence of cholesterol sulfate in prostate tissues might serve as a tool for prostate cancer diagnosis although confirmation through larger and more diverse cohorts and correlations with clinical outcome data is needed.

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