Crude oil-polluted sites are a global threat, raising the demand for remediation worldwide. Here, we investigated a crude oil metagenome from a former borehole in Wietze, Germany, and reconstructed 42 metagenome-assembled genomes, many of which contained genes involved in crude oil degradation with a high potential for bioremediation purposes.

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Ethanol addition to diesel fuels significantly affects the leaching potentials of petroleum hydrocarbons, thereby making them inaccessible to rhizoremediation.