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Space-related activities remain a fertile environment for classroom applications of chemistry through both traditional lecture styles and through collaborative learning projects. With the resurgence of lunar exploration and the expansion of the commercial space sector, serious scholarship, planning, and resources have been focused on extracting and using lunar materials to sustain activity in space. In this work, several interdisciplinary general chemistry topics and assessment activities are explored through the lens of lunar resource formation, identification, extraction, purification, and employment. Four applications of chemistry are presented: the formation of lunar water via reduction of ores by solar wind, identifying likely locations of lunar water via spectroscopy, extracting water from the lunar regolith for use as fuel, and the extraction, purification, and use of materials from the lunar regolith. A variety of traditional general chemistry topics are explored through these applications, to include light, energy, unit conversions, equilibrium, phase diagrams, oxidation–reduction, and semiconductors.