Abstract:This paper introduces a disposable battery-driven heating system for loop-mediated isothermal DNA amplification (LAMP) inside a centrifugally-driven DNA p urification platform (LabT ube). We demonstrate LabT ube-based fully automated DNA purification of as low as 100 cell-equivalents of verotoxin-producing Escherichia coli (VT EC) in water, milk and apple juice in a laboratory centrifuge, followed by integrated and automated LAMP amplification with a reduction of hands-on time from 45 to 1min. The heating system consists of two parallel SMD thick film resistors and a NT C as heating and temperature sensing elements. They are driven by a 3V battery and controlled by a microcontroller. The LAMP reagents are stored in the elution chamber and the amplification starts immediately after the eluate is purged into the chamber. The LabT ube, including a microcontroller-based heating system, demonstrates contamination-free and automated sample-to-answer nucleic acid testing within a laboratory centrifuge. The heating system can be easily parallelized within one LabT ube and it is deployable for a variety of heating and electrical applications.
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