In this paper, the authors present the study of spin-labeled poly(l-lysine) biological molecules (polypeptides) using electron paramagnetic resonance, atomic force microscopy, and electron spin noise-scanning tunneling microscopy. In the presented studies, the authors were able to obtain the information about the local distribution of the spin signal emission, and they registered the spectra for the selected area with nanometer resolution. The preparation method of spin-labeled biopolymer, experimental setup, and the procedure of calibration are also described in detail.

aThe studies of (TEMPO) 3 O 3 P molecules (tri-(2,2,6,6-tetramethyl-1-oxyl-4-piperidyl) phosphite) and their monolayers formed on highly oriented pyrolytic graphite (HOPG) are presented. (TEMPO) 3 O 3 P is synthesized from TEMPOL precursor (4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl). We focused on electron paramagnetic resonance (EPR) and scanning tunnelling microscopy (STM) investigations. The EPR experiments demonstrate the paramagnetism of (TEMPO) 3 O 3 P in powdered form and in solution. Furthermore, these paramagnetic properties of (TEMPO) 3 O 3 P are preserved after the deposition on HOPG substrate. The STM studies show that (TEMPO) 3 O 3 P molecules have a strong tendency to form self-assembled monolayers (SAMs). The detailed STM images let us to explore the structure of (TEMPO) 3 O 3 P on HOPG and propose a structural model for the observed SAMs.