No abstract

To compare the level of contact with customers, 4 ways of contacting them were selected. Adverse developments showed up for the customer records, higher was its randomness, which was not expected. More sophisticated ways of contacting customers confirmed a positive change, though not very significant. The most important result in 2005 and 2015 was the increase in the number of loyal customers, which contributes to the growth of profitability. In the end of the article, hypothesis about the relationship of the size of SMEs and CRM knowledge is confirmed by using logistic regression, and premises are verified about the comparison of results in various years. It turns out that the CRM can be beneficial for SMEs, so it should be implemented in its management system.

The article deals with the issue of using strategic marketing in the business of small and medium-sized enterprises. The main objective of this paper is to theoretically define the principles of strategic marketing and then practically analyse the level of strategic marketing use as a tool of business management in selected Czech small and medium-sized enterprises. In order to fulfil the research plan, secondary and primary research was carried out. The theoretical part defines the basic problem areas such as various approaches to strategic marketing defining and strategic management process versus strategic marketing process. In the practical part, 335 companies are analysed, the results are commented and hypotheses evaluated. The most important findings are generally low rate of utilization of strategic marketing management tools and the utilization of these tools is dependent on company size, with micro companies being the worst and medium companies the best.

Genetically identical cells are known to exhibit differential phenotypes in the same environmental conditions. These phenotypic variants are linked to transcriptional stochasticity and have been shown to contribute towards adaptive flexibility of a wide range of unicellular organisms. Here, we investigated transcriptional heterogeneity and stochastic gene expression in P. falciparum by performing single cell RNA sequencing on blood stage schizonts. Our data reveals significant transcriptional variations in the schizonts stage with a distinct group of highly variable invasion gene transcripts being identified. Moreover, our data reflected several diversification processes including putative developmental “checkpoint”; transcriptomically distinct parasite sub-populations and transcriptional switches in variable gene families (var, rifin, phist). Most of these features of transcriptional variability were preserved in isogenic parasite cell populations (albeit with a lesser amplitude) suggesting a role of epigenetic factors in cell-to-cell transcriptional variations in human malaria parasites. Lastly, we applied quantitative RT-PCR and RNA-FISH approach and confirmed stochastic expression of merozoites surface proteins encoding msp1, msp3, msp7, rhoptry protein encoding rhopH2 and erythrocyte binding antigen encoding eba181, some of which represent key candidates for invasion blocking vaccines.

Cílem tohoto článku je prozkoumat teoretické poznání řízení vztahů se zákazníky (CRM) a jeho princip fungování, aby mohly být nalezeny oblasti, kterými se autoři budou zabývat v navazujícím primárním výzkumu. Proto tento článek zkoumá vývoj definic CRM, od jejich úplných počátků v 90. letech 20. století po současnost. Na tomto vývoji autoři popisují, jak se měnil přístup k CRM. Dále článek popisuje aplikační architekturu CRM, tedy analytické, operativní a kolaborativní CRM, a uvádí příklad jejich fungování v praxi. Autoři také zmiňují výhody a nevýhody, které CRM přináší. Poslední část článku je věnována zamyšlení nad situací malých a středních podniků, zda tyto mají vůbec možnost CRM implementovat. V závěru článku autoři shrnují klíčové body vystupující z poznání teorie, které by měl obsahovat dotazník.