Silver doped ZnO films was prepared by the sol-gel and dip-coating technique, starting with zinc acetate and silver acetate as precursors, followed by its hydrolysis in ethanol. Acetic acid was incorporated to adjust pH, as well as ethyleneglycol and Triton MR as stabilizers. The sol was later dipped 3 times in Corning glass. Structural, morphological and antimicrobial properties of the films were investigated for three silver contents (1.0, 2.5 and 5 mol %). X-ray diffraction (XRD) shows that the films have a hexagonal structure after been annealed at 500 °C. Atomic force microscopy (AFM) for films showed a homogeneous, crack free and smooth surface, composed of cross-linked particles. The synthetized films presented antibacterial activity against Escherichia Coli. It was observed that the film with the higher Ag content (5 mol %) presents the higher antimicrobial activity at 72%.