A number of pseudomonad bacteria were investigated for their in vitro antagonism on agar against Pseudomonas tolaasii, the causative agent of bacterial blotch of the cultivated mushroom. Addition of FeCl3 to the culture medium suppressed the antagonism in 14 of the 20 bacteria tested and the production of a substance with an absorption peak at 400 nm by all antagonists was eliminated by the presence of Fe3+. Both observations indicated that siderophores could be involved in the mode of action of some antagonists. The addition of the iron chelators EDTA and bipyridyl to the medium used for culture of antagonists and pathogens did not generally prevent growth. Siderophore production is not essential for the in vivo activity of antagonists.

No abstract