Astrocytes are a target for regenerative neurobiology because in brain injury their phenotype arbitrates brain integrity, neuronal death and subsequent repair and reconstruction. We explored the ability of 3D scaffolds to direct astrocytes into phenotypes with the potential to support neuronal survival. Poly-e-caprolactone scaffolds were electrospun with random and aligned fibre orientations on which murine astrocytes were sub-cultured and analysed at 4 and 12 DIV. Astrocytes survived, proliferated and migrated into scaffolds adopting 3D morphologies, mimicking in vivo stellated phenotypes. Cells on random poly-e-caprolactone scaffolds grew as circular colonies extending processes deep within sub-micron fibres, whereas astrocytes on aligned scaffolds exhibited rectangular colonies with processes following not only the direction of fibre alignment but also penetrating the scaffold. Cell viability was maintained over 12 DIV, and cytochemistry for F-/G-actin showed fewer stress fibres on bioscaffolds relative to 2D astrocytes. Reduced cytoskeletal stress was confirmed by the decreased expression of glial fibrillary acidic protein. PCR demonstrated up-regulation of genes (excitatory amino acid transporter 2, brain-derived neurotrophic factor and anti-oxidant) reflecting healthy biologies of mature astrocytes in our extended culture protocol. This study illustrates the therapeutic potential of bioengineering strategies using 3D electrospun scaffolds which direct astrocytes into phenotypes supporting brain repair.

In the context of complex intergenerational challenges such as climate change and sustainable development, it is increasingly important for scientists and policy-makers to actively engage with and support the meaningful participation of youth in policy and decision-making. This research evaluates the effectiveness and impact of the Youth in Landscapes Initiative in supporting the active participation and leadership development of youth (aged 18–30 years old) participants at the 2014 Global Landscapes Forum (GLF), an international science-policy forum. This ‘youth program’ comprised a Youth Session, skills workshops and mentoring programs to empower youth through leadership and capacity building opportunities. Results show a high demand for youth participation: 34% of GLF conference delegates expressed interest to attend the Youth Session, over 22% of GLF session organisers requested youth to take on leadership roles, and the youth program itself received over 770 applications for the ‘facilitator’ and ‘pitcher’ leadership positions. The skills-based ‘masterclasses’ successfully built the confidence and knowledge of youth participants, as shown by post-evaluation survey responses. This translated into active and substantive youth participation throughout the forum. Senior professionals connected to the program praised it highly, seeing it as an opportunity for mutual, intergenerational learning. The Youth in Landscape Initiative is presented as a model and distilled into a framework to inform future youth engagement strategies in international conferences and associated science-policy processes.